Fig. 5-5. PAGE activity staining of purified SP-I 

 and SP-II. SP-I was added to lanes 2 and 3, and SP-II 

 was added to lanes 1 and 4. Activity staining was done 

 under optimal conditions. A) After incubation for about 

 15 min, SP-I showed two bands of activity in both lanes. 

 SP-II, at lower concentrations, showed two bands in lane 

 4, each migrated faster than the SP-I bands. B) The gel 

 was incubated further for about 15 min. The SP-II in lane 

 1 was then visible. C) After incubation for 1 h, 2 bands 

 were more pronounced for all four lanes. 



