Ill 

 antibody showed maximal inhibition of both proteins with 

 about 100/il antibody (Fig, 6-2) . 



Crude extract was applied to several wells of a native 

 gel. After electrophoretic migration, the gel was divided. 

 One half of the gel was used for Western blotting with Sl- 

 antibody and the other half of the gel was used with SDH 

 activity stain. Figure 6-3 shows the visible bands for 

 each. 



When SP-I specific antibody was incubated with 

 E. coli crude extract and N. silvestris crude extract and 

 then assayed for SDH activity, (Table 5-1) the E. coli SDH 

 activity was retained, while the plant SDH was precipitated 

 and activity was lost. 



