Fig. 7-1. Agarose gels of cDNA encoding aromatic 

 pathway proteins. A) The cDNA inserts in pBluescript 

 are shown. B and C) Restriction enzymes (all found in 

 the multiple cloning site of pUC 18, pGEM or pBluescript 

 plasmids) were individually used to determine the number 

 of RE sites (or fragments) of the cloned cDNA insert in 

 pBluescript encoding the S-protein: 



(left) 



B 



(right) 





cDNA 



Restriction 



Restriction 



Lanes 



Clones 



Enzyme 



Sites 



Enzyme 



Sites 



1 



PATS 



Hindi I I 



3 



Sail 



1 



2 



SPIO 



Sad 







BamHI 







3 



SP33 



Kpnl 







Ndel 



1 



4 



SP3 



SP3 



- 



Spel 







5 



PAT7 



STD 



- 



Banll 



3 



6 



SP5 



Xbal 







SP3 



- 



7 



STD 



Xhol 







STD 



- 



8 



pBluescript 



Not I 







EcoRV 



3 



9 



SP6 







EcoRI 



1 



10 



ADHl 







Smal 



1 



11 



ADH4 







PstI 







12 



ADH8 







BbuI(Sphl) 



1 



