Fig. 7-4. Agarose gels of SP3 cDNA fragments during 

 stages of subcloning. SP3 fragments were visualized on 

 agarose gels after incubation with restriction enzyme (s) . 

 A) The first three lanes contained cDNA fragments cut 

 with Hindlll (from 500 to about 800 bp) , the second set 

 of three lanes contained cDNA fragments cut with Sphl and 

 Sail (about 300 and 1100 bp) and the last set of three 

 lanes contained fragments cut with Banll (about 500,700 

 and 900 bp) . B) The first three lanes contained cDNA 

 fragments cut with Ndel and EcoRV (about 300, 500 and 900 

 bp) . C) All lanes show that plasmids carrying the cDNA 

 fragments were transformed into competent cells. The 

 lowest bands represent RNA, the mid bands are the plasmid 

 DNA bands and the top bands are genomic DNA. D) Lanes 

 contain purified plasmid DNA containing cDNA fragments. 

 Bands are of different sizes depending on the size of the 

 ligated fragment. 



