145 



Table 7-3. NAD{P)H Binding Motif of Enzymes'. 



Organism 



cDNA or Gene Product 



Conserved Region 



GxGxxGG 



GxGxxVxxxA Accession 



GxGxxAxxxA Number 



E. nidulans 



N. crassa 



E. nidulans 



N. crassa 



E. nidulans 



S. cerevisiae 



E. coli 



E. coli 



N. tabacum 



S. tuberosum 

 N. tabacum 

 N. tabacum 

 H. vulgare 

 T. repens 



C. roseus 

 T. aestivum 

 N. silvestris 



H. sapiens 

 H. sapiens 

 M. musculus 



P. stipitis 



S. cerevisiae 



K. marxianus 



C. synechocystis 



Quinate repressor (QutR) 

 Quinate repressor (Qa-lS) 

 Quinate dehydrogenase (QutB) 

 Quinate dehydrogenase (Qa-3) 

 Shikimate dehydrogenase (AroM) 

 Shikimate dehydrogenase (Arol) 

 Shikimate dehydrogenase (AroE) 

 unidentified reading frame 

 S-protein {AroD»E) 



Malate dehydrogenase 

 Cinnamyl -Alcohol dehydrogenase 1 

 Cinnamyl- Alcohol dehydrogenase2 

 Alcohol dehydrogenasel 

 Alcohol dehydrogenasel 



S-Adenosylhomocysteinase 

 S-Adenosylhomocysteinase 

 S-Adenosylhomocysteinase 



Alcohol dehydrogenase gamma 

 Glutathione reductase 

 Lactate dehydrogenase 



D-Xylulose reductase 

 Succinate dehydrogenase 

 Alcohol dehydrogenasel 

 Ketolate reductoisomerase 



STTGLIIGAGGMARAGIYAM M59935 



TSTGLVIGAGGMARAAVYAM P11637 



GKPALIVGGGGTARTAIYVL P25415 



SRPALVIGGGGAARSAVYAL P1163 5 



EQSALWGGGGTARAALYAL P07547 



HTAGLVIGAGGTSRAALYAL P08566 



GLRILLIGAGGASRGVLLPL P15770 



GKTMVLLGAGGASTAIGAQG P28244 

 GKLFWIGAGGAGKALAYGA 



KMKIWA6AGSAGIGVLNAA Z23023 



GFRGGILGLGGVGHMGVKIA P3 0360 



GLRGGILGLGGVGHMGVKIA P30359 



GSTVAIFGLGAVGLAAAEGA P05336 



GSSVAIFGLGAVGLAAAEGA P13603 



GKVAWAGYGDVGKGCAAAL P35007 



GKVAWCGYGDVGKGCAAAL P32112 



GKVALVAGYGDVGKGCAAAL D16138 



GSTCAVFGLGGVGLSWMGC P00326 



SYDYLVIGGGSGGLASARRA S08 979 



NNKITWGVGQVGMACAISI P16125 



GDYVAVFGAGPVGLLAAAVA P22144 



WIGAGGA6LRAAFGL Q00711 



TKLPLVGGHEGAGVWAMGE P 2 3 6 9 



GKTVAIIGYGSQGHAHALNL A4703 7 



E. coli 



Threonine dehydrogenase 



E. coli 



Glutamate synthase 



T. brucei 



Phosphogluconate DH 



T. brockii 



NADH oxidase 



P. putida 



Formaldehyde DH 



GEDVLVSGAGPIGIMAAAVA P07913 



GKKVAIIGAGPAGLACADVL B2 9617 



WGLGVMGANLALNI P31072 



KKWWGGGPAGMQAAITA P32382 



GSTVYVAGAGPVGLAAAASA D21201 



"Motifs were taken from Scrutton et al . , 1990. The first nine set of 

 organisms are those used in the SDH multiple allignment of Fig. 7-8. 



:d 



