136 Proceedings. 



the xylem parts of the conducting bundles. Among 

 the tissues so differentiated are : — (a) the phloem of 

 the conducting bundles, (b) the living cells of the 

 cortex, pith, medullary rays, xylem parenchyma, &c. 

 Of the more delicate structures, chorophyll corpuscles, 

 leucoplasts, and sometimes the nuclei, are stained of a 

 deeper blue than the cytoplasm of the cell, and can be 

 clearly distinguished from it, while the red stains the 

 cuticular layers of the epidermal cells, the walls of cork 

 cells, the thickening layers of the endodermis, the con- 

 tents of resin and tannin cells, and the granules of starch, 

 at least in some cases. Lastly, in well stained preparations, 

 several details of the complex structure of the nucleus are 

 often brought out by this method of treatment, though 

 not perhaps in all cases. Thus in many instances we can 

 distinguish, by their red colour : — {a) the chromatin granules 

 or threads in the linin threads, (b) the nucleolus, and (c) 

 the karyokinetic figures of the dividing nucleus, from the (d) 

 nuclear wall which is blue, and (e) the nuclear sap which is 

 colourless. Among the many plant structures that are 

 specially suitable for illustrating the effects producible by 

 this method of double staining, the following may be recom- 

 mended as easily accessible and requiring no exceptional 

 treatment : — i. Rhizome of Pleris aquilina ; ii. Stem of 

 Lycopodium clavatum ; iii. Root of Phoenix dactylifera ; 

 iv. Root of Vicia Faba ; v. Leaf of Pinus pinaster ; vi. Stem 

 of Cyperus; vii. Stem of Ranunculus repens ; viii. Tuber of 

 Phajus maculata ; ix. Root of Abies ; x. Anther of Lilium 

 croceum in young condition." 



Mr. Oldham read an extract from " A brief History of 

 the Hawaiian People," by W. D. Alexander, New York, 

 page 89, describing the game played with the rounded 

 stones exhibited at the preceding meeting. He also 

 exhibited a specimen of Stenopteryx hirundinus, parasitic 

 on the swift. 



