2 J. W. W. STEPHENS^METHODS FOR DETECTING SPOROZOITS 



careful examination with a pocket-lens should enable you to state almost with 

 certainty whether or no all the Anophelines you have caught are of the same 

 species. Retain say a dozen, including some males, for examination by an 

 expert. Kill them in the test tube by placing a drop of chloroform on the plug. 

 Drop them on to a layer of very loosely fluffed cotton-wool in a pill-box or 

 match-box. Place a very thin layer of loosely fluffed cotton-wool over them to 

 prevent shaldug. If obtainable, add one drop of creosote to the inside of the 

 lid of each box to prevent moidd, or melt a little naphthaline and pour it into the 

 lid. 



Put a label outside the box stating when and where caught and naine of sender. 

 Dispatch home at once. 



Dissection of a Mosquito. 



Killingf- — This is most easily done by concussion, i.e. rapping the tube, with 

 the mosquito at the end, smartly against the knee. (N.B. — This must on no 

 account be done if the mosquitos are required for identification, as most of the 

 scales are knocked off in the process). 



Trimming. — Put the mosquito on a slide, and while holding one wing pull off 

 with a needle or forceps the legs and remaining wing. Take cave not to pull 

 off the proboscis by mistake. 



Dissection of Salivary Glands. 



(«.) Put the trimmed mosquito into a drop of salt solution (1 per cent, roughly) 

 on a slide so that it lies on its rifjht side with its proboscis towards you (fig. 1), 



Fig. 1. — Diagram to illustrate the method of extracting the salivary glands of a mosquito.* 



*■ This drawing has been kindly made for me by Mr. H. F. Carter, Assistant Entomologist, 

 Liverpool School of Tropical Medicine, 



