6 j. w. w. stephens — methods fok detecting sporozoits 



Parasites that may be met with during Dissection. 



1. Trematodes — encysted in the thorax, also free in the stomach. 



2. Nematodes^in the thorax or abdominal cavity. In the thoracic muscles, 

 filarial larva3 may also be fomid. 



3. Sporozoa— (a) masses of sausage-shaped bodies about the salivary glands ; 

 (b) sporocysts containing 8 spores — these occur in large numbers replacing the 

 yelk of the eggs (Fig. 5) ; (c) gregarines, free in the stomach or encysted in 

 the Malpighian tubes. 



■7 »^.'^.>.j^ 





Fig. 5.^Protozoa other than the malarial parasite fouuJ in Auophelines. 



4. Flagellata — in large numbers in the gut. They are Crithidia-ioi:ms. 



5. Micro-organisms and Nosema (?) in the diverticula. 



6. Ecto-parasites — The reddish larval stages of water-mites (Hydrachnidae) 

 are common. The adult stages of these are unknown. 



Infection of Anophelines. 



Our information on this subject is deplorably inadequate. A systematic 

 examinati(.'n of suspected species at all seasons of the year would certainly yield 

 results of considerable practical value. The following are examples of some of 

 the results already obtained (Stephens and Christophers) : — 



Species of Percentage infected 



Anopheline. with sporozoits. 



Fyretophorus costalis ... 3 



Mijzomyiu junesta ... 50 



My zomyia juneita ... 25 



ij Myzomyia culicifacies , . , 4'6 



\Myzomyia rossii ... 



^ Myzomyia culicifacien... 8*6 



\ Myzomyia rossii ... 



Nor should the Culicines be neglected in this I'espect. An investigation of 

 the commoner house-frequenting species might yield important information. 



Locality. 



Lagos Town ... 

 Olokemeji (S. Nigeria) 

 Aro (S. Nigeria) 



Mian Mir (Punjab) ... 

 Ennur (Madras) 



