lo interest for systamsftic bo 
= 6] This recognition of the applicability of sero-diagnosis to botany, however, 
A continued. to be without further importance for our knowledge of the historical de- 
-= „velopment of the plant-kingdom until MEZ, starting from the highest dicotyle-fa- 
milies, began working through the whole plant-system in a collective research oar- 
Tied out in collaboration with his students in the Botanical Institute at Kónigs- 
berg from 1911 onwards. By means of it, the Experimental Systematical Method was 
produced as a special branch of Science. The results of this research that have 
been gained up to the present ere submitted in the "Königsberger Stammbaum des 
 Pflanzonroeichoes". 
| 7) The method of examination was at first the one taken from medical science. 
Rabbits were employed for immunization; they were inoculated with the extracts of 
plants, after attaining sufficient immunity, were killed, The blood-serum was ba- 
ken and mixed with extracts of the plants which were to be tested for their rela- 
_tionship. Existing relationship shows itself through the appearence of specific 
‘precipitates. These serums are called natural seruns; they are now frequently re- 
| placed by the artificial serums discovered by us, which are quite identical as re- 
gards their effect. 
8) While medical research in general presumes even to-day that the "immunity- 
Substances" are produced as the reaction of the body of the living animal on the 
foreign proteinous substances injected, that they are substances which are produ- 
ced by the animal body, we have shown that living animals are unnecessary for its | 
production and that the immunity serums can be produced just as well or better P ri 
digestion in vitro of the proteinous substances with serum of animals which h — 
7 killed (artificial serums). The dead blood-serum has also the property of 
= take dawn the proteinous substances by fermentation, during the production 
8 immunity substances. 
5 t was sh&wn My us that en living organs of every species yield the samo 
ener G Inmmur Ranges in all stages of er ar {youth and age, 
Haploid- and Diloid-Phase]. c consequont me-sacific" character of the immuni- 
ty-substances is settled by the specific Sharaa tar bor the "spec — AE 
GE Zeile" 6. HERTE TEIG). ; 
10) As parts of animals, free of nuclei as well as chromatinous substances 
yield no immunity-substances which are typical for species, it is extremely pro- 
 bable that the property of yielding immunity-substances for specifying relation- 
Ship belongs to the substances of tho nuclei. Through this proposition the Expe- 
rimental Systematical Method gains accession to the views of the investigators 
: of heredity and represents a branch of Genetics which concerns the whole system. 
11) Just as genetics assume with certainty that all tha properties of the or- 
rp gani sms are handed down to posteriori ty by physical transmission of the heredita- 
ry substances in the process of propagation, it is also the logical basis of the 
Experimental Systematical Method that the proteinous substances (which are ap- 
parently localised in the nucleus] and their properties (Idioplasma) are the cau- 
88 of &ll morphological and physiological shapos of the organisms. 
412) Prom similar or dissimilar characteristics, it is generally concluded ` 
Sher on organisms are related or not. The same conolusion must also be ee 
th regard to the similarities and dissimilarities which appear in the La 
reactions. 4 
5) These accords and divergences of the reactions ne by berge 
had led to a system obtained by experiment, which agree "fe 
nes Of historical-phylogenetical research (Palaeontology, pue of deve 
) in ‘very conne 3 coincides with the views of the best, throug! 
