40 The Philippine Journal of Science m& 



Table II), type Giles showed a fair percentage of eases with 

 well-developed ova at dissection, while type indefinitus showed 

 practically none, comparing insects of the same age at dissection. 



Experimental case 408, male, 33 years of age and formerly a 

 mining coolie, was born in China and had been in the Federated 

 Malay States four years. He had no history of fever. He had 

 had beriberi three years previously and still had the beriberi 

 gait, but was well nourished and otherwise in good physical 

 condition. The spleen was not enlarged. No parasites were 

 found in the blood for seventeen or more successive daily exam- 

 inations. On March 20 the mosquitoes remaining from the lot 

 used in case 727 were exposed to No. 408, but none bit, although 

 they had not been given food for five days. On the same date 

 seven of another lot were tried, and none bit. On March 21 the 

 four of the seven remaining alive were tried and two bit. The 

 first bit feebly and took only a small quantity of blood. The 

 second was observed to bite twice, at least, and took a fair amount 

 of blood. On dissection the first showed no parasites, but the 

 second had one empty oocyst in the gut and sporozoites in the 

 salivary glands. Of another lot of three tried on the same day 

 one bit. It had oocysts, apparently degenerate, in the mid-gut, 

 but no sporozoites in the salivary glands. So it seems clear that 

 the subject was bitten by only one sporozoite-infected mosqui- 

 to and that this one bit at least twice. This mosquito was also 

 infected from crescent carrier 537 (Table XI) sixteen to sixteen 

 and one-half days previously. The lot of mosquitoes were ex- 

 posed twice to this carrier on succeeding days. At the first 

 exposure the percentage of crescents was 5.1, at the second, 1.7. 



Various observations on these two experimental cases are com- 

 pared in Table XVII. 



No. 727 showed no rise of temperature until some eight hours 

 after parasites were found in the blood, while no parasites were 

 found in No. 408 until the third day after the rise of temperature. 

 No. 727 showed marked symptoms, headache, severe vomiting, 

 and on the third and fourth days after illness began, severe 

 attacks lasting about twenty minutes characterized by convulsive 

 symptoms, clutching of the fingers, pain in the throat, and rapid 

 and shallow respiration. The spleen was not enlarged at any 

 time in this case. No. 408 had practically no symptoms at 

 all, except that he complained of headache on one day. There 

 was marked enlargement of the spleen. Both cases made a rapid 

 and complete recovery under quinine treatment. Temperature 

 charts of both cases are given. 



