96 The Philippine Journal of Science 191s 



The drugs used in the following experiments were as follows : 

 (1) Eosin; (2) medicinal methylene blue (Merck) ; (3) cacody- 

 lateof soda; (4) atoxyl; (5) quinine sulphate ; (6) camphorated 

 oil; (7) creolin; (8) permanganate of potash; (9) ergot; (10) 

 iodine; (11) potassium iodide; (12) gentian violet; (13) 

 adrenalin hydrochloride; (14) nuclein; (15) formalin; (16) 

 chlorazene; (17) castor oil; (18) alcohol; (19) fluid extract of 

 nux vomica; and (20) fluid extract of gentian. 



With the small amount of experimentation that has been 

 given to each drug, no promising results have been obtained 

 by the method in which they were administered and the dosage 

 in which they were given. 



In all the experiments where sodium chloride solution was 

 made use of to dilute the drug for intravenous and intraperi- 

 toneal injections, 0.85 per cent was used. It was found when 

 giving large intravenous and intraperitoneal injections that if 

 the fluid was warmed to about 41° C. the animals withstood the 

 injections with much less discomfort than when the solutions 

 were cooler. All the large intravenous injections were per- 

 formed in the manner illustrated in Plate I. 



EOSIN 



Eosin was used with the idea that it might have a special 

 affinity for the virus of rinderpest, at it is one of the diffuse 

 stains and penetrates well. 



EXPERIMENT 1 



Batanes bull 3153, which had contracted rinderpest by expo- 

 sure to sick animals and had run a high temperature for forty- 

 eight hours, was injected subcutaneously on the afternoon of 

 May 30, 1911, with 2 grams of Grubler's W. Gelb eosin dissolved 

 in 100 cubic centimeters of sterile distilled water. 



July 1 this animal's temperature subsided to normal, but it 

 developed inappetence and diarrhoea and died July 3, presenting 

 good lesions of rinderpest upon autopsy. The subcutaneous and 

 mesentery tissues had taken on a pinkish coloration, resulting 

 from the free distribution of eosin throughout the body, which 

 apparently had no ill effects upon the virus of rinderpest. 



MEDICINAL METHYLENE BLUE 



Methylene blue was used in experiments 2 and 47 with the 

 idea that if perchance the virus of rinderpest was an intracor- 

 puscular organism this dye might have a direct action upon it, 

 as is the case in malaria. Its antipyretic and anodyne actions 

 were also considered. 



