xin, b, 2 Boynton: Treatment of Rinderpest 97 



EXPERIMENT 2 



Batanes bull 3116 was injected on May 23, 1911, with 30 cubic 

 centimeters of filtered blood handled as follows: Five cubic 

 centimeters of virulent rinderpest blood from bull 3135 was 

 diluted up to 500 cubic centimeters with sterile 0.85 per cent 

 sodium chloride solution; this diluted blood was then passed 

 through a Berkefeld N. filter under 3 kilograms' pressure. 



This animal presented its first rise in temperature on the 

 morning of May 28, registering 39.2° C. 



During the afternoon of May 29, or thirty-six hours after 

 the first rise in temperature, this animal was injected subcu- 

 taneously with 2 grams of Merck's medicinal methylene blue 

 dissolved in 100 cubic centimeters of sterile distilled water. 



May 30 this animal's urine was dark blue. Its visible mucous 

 membranes also took on a bluish coloration. 



May 31, diarrhoea, eating little. 



June 1-3, diarrhoea, not eating. 



June 4, died, presenting good lesions of rinderpest upon 

 autopsy. 



The methylene blue thus administered had no apparent ill 

 effect upon the virus of rinderpest. 



CACODYLATE OF SODA 



Cacodelate of soda was tried with the idea that it might have 

 an action upon the virus of rinderpest similar to that which it 

 has upon Treponema pallidum, although arsenobenzol (salvarsan, 

 606) has been tried on rinderpest with negative results. (The 

 reference to the experiments with salvarsan cannot be located.) 



EXPERIMENT 3 



Carabao 3088 had contracted rinderpest by exposure to sick 

 animals. 



May 17, 1911, the fourth day of temperature, this animal was 

 injected intravenously in the femoral vein with 6 grains of 

 cacodylate of soda. The animal's temperature subsided to nor- 

 mal in one and one-half days after the injection, but it suffered 

 severely from photophobia; diarrhoea, not eating. 



The animal died the night of May 19, presenting marked 

 lesions of rinderpest. 



EXPERIMENT 4 



Batanes bull 3158. — This animal had been injected with viru- 

 lent rinderpest blood. 



May 19, 1911, which was the second day of temperature, this 

 animal was injected intravenously in the femoral vein with 5.25 



