104 The Philippine Jouimal of Science 1918 



EXPERIMENT 15 



This experiment was made to test the infectivity of blood of 

 an animal that had been given a 3 per cent creolin solution 

 intravenously. 



Batanes bull 331U. — December 6, 1911, this animal was in- 

 jected with 25 cubic centimeters of blood taken from carabao 

 3183, twenty-four hours after this carabao had been injected 

 intravenously with 1,000 cubic centimeters of a 3 per cent creo- 

 lin solution. This injection was made to ascertain whether or 

 not the virus had been killed in the blood by this heavy in- 

 jection of creolin. 



December 9, this animal presented a rise in temperature, 

 registering, in the forenoon, 39.2° C. ; in the afternoon, 40.1° C. 



December 11-12, eating little. 



December 13-14, diarrhoea, eating little. 



December 15-21, diarrhoea, not eating. 



December 22-23, bloody diarrhoea, not eating. 



December 24, this animal died, presenting good lesions of 

 rinderpest upon autopsy. 



This proves that the virus was in virulent form in the blood 

 of carabao 3183 at the time it was drawn and that the creolin 

 had apparently no detrimental effect upon the virus. 



PERMANGANATE OF POTASH 



Like creolin, permanganate of potash was tried for its anti- 

 septic effect upon, and its possible attenuation of, the virus. 

 Walker (3) has used permanganate of potash on animals sick 

 with rinderpest and has obtained some favorable results. How- 

 ever, he was working with animals having a high natural im- 

 munity to the disease, which undoubtedly accounts for much of 

 his success. 



EXPERIMENT 10 



Batanes bull 3307. — This animal was injected on November 

 22, 1911, with virulent rinderpest blood that had been kept in 

 a test tube in a clotted form for forty-eight hours at 37° C. 



November 27, this animal presented its first rise in temper- 

 ature, registering, in the forenoon, 39.7° C; in the afternoon, 

 40.6 C C. 



November 29, diarrhoea beginning. 



November 30, diarrhoea, not eating; administered subcuta- 

 neously 900 cubic centimeters of a 1-2,000 solution of permanga- 

 nate of potash in physiological salt solution. 



December 1-2, diarrhoea, not eating. 



