108 The Philippine Journal of Science 1918 



July 16, administered intravenously 1,000 cubic centimeters 

 of sodium chloride solution to which 2.5 cubic centimeters of 

 40 per cent formalin had been added. 



July 17-20, diarrhoea, not eating; very sick; blood in fceces; 

 mucopurulent discharge from nostrils and eyes. 



July 20, died, presenting good lesions of rinderpest. 



EXPERIMENT 26 



Fuga carabao 132. — Contracted rinderpest by exposure to 

 sick animals. 



July 14, 1917, this animal presented its first rise in temper- 

 ature, registering, in the afternoon, 40° C. 



July 15, diarrhoea, not eating. 



July 16, diarrhoea, not eating; administered intravenously 

 1,000 cubic centimeters of sodium chloride solution to which 

 2.5 cubic centimeters of 40 cent formalin had been added ; with- 

 stood injection well. 



July 17-18, diarrhoea, not eating; very sick. 



July 18, died presenting good lesions of rinderpest. 



In the seven experiments just described, it will be noted that 

 one animal, Dalupiri carabao 3182, experiment 20, recovered 

 from rinderpest. From the results of the six other experiments 

 it may be granted that the administration of formalin played 

 no part in the recovery, as this animal would undoubtedly have 

 recovered without any treatment, as many animals do. 



GENTIAN VIOLET 



Gentian violet was used in this experiment with the idea that 

 it might have some direct action upon the virus. Russell (2) has 

 found in his research work that gentian violet has a direct 

 action upon protozoa in high dilution, but has no detrimental 

 effect upon tissue growing in vitro. 



EXPERIMENT 27 



Batanes bull 3931 was inoculated with rinderpest culture on 

 July 12, 1915. 



July 16, this animal presented its first rise in temperature. 



July 19, administered intravenously 1,000 cubic centimeters 

 of sodium chloride solution that had 2 grams of gentian violet 

 dissolved in it; took injection well. 



July 21-22, not eating. 



July 22, died, presenting good lesions of rinderpest, showing 

 that the virus was not affected by the injection. 



