xiii, b, 3 Boynton: Virulence of Body Organs in Rinderpest 129 



ceptible. They were obtained from localities where, to our 

 knowledge, rinderpest has never been introduced, or from local- 

 ities where the presence of rinderpest has not been known for 

 many years. These animals were brought to the laboratory 

 and placed under observation for various lengths of time, which 

 will be mentioned in each experiment. During the period of 

 observation in the quarantine shed and throughout the course 

 of the experiments their temperatures were taken twice daily 

 and their general appearance was noted. The animals were 

 kept in an isolation shed while under experimentation and until 

 the first symptoms of disease appeared. Usually one or more 

 susceptible animals were among them to check up any accidental 

 infection that might gain entrance from sources other than by 

 inoculation. As soon as the first symptoms of disease made 

 their appearance, the animal thus affected was immediately 

 transferred to the shed where the sick animals were kept. 



The following abbreviation will be used in the experiments: 

 P. C. W., animals used by the Philip C. Whitaker antirinder- 

 pest serum plant. 



EXPERIMENT 1 



Water extract of liver, spleen, and lymph glands, 3 days old. 



Carabao 69. — Known history prior to the experiment : Native 

 Fuga carabao, 3 years old, received at the laboratory and placed 

 in quarantine January 7, 1917. This animal was kept under 

 observation for twelve days before it was used and at no time 

 during this period did it present a high temperature or develop 

 any symptoms of sickness. 



February 6, 1917, carabao 69 was injected subcutaneously 

 with 100 cubic centimeters of a 3-day-old water extract from 

 the liver, spleen, and lymph glands of carabao 66, which was 

 bled to death on the third day of temperature for virulent blood, 

 to be used in immunization work. Fifty grams of each of these 

 tissues were used, and 300 cubic centimeters of water were added 

 and allowed to extract in the refrigerator. After three days 

 of extraction the material was filtered through gauze, and 100 

 cubic centimeters of the filtrate were used in this experiment. 



February 10, carabao 69 presented an afternoon temperature 

 of 40.5° C. 



February 11, forenoon temperature, 40.3° C. ; afternoon tem- 

 perature, 40.8° C. ; diarrhoea, not eating. 



February 12-13, diarrhoea, not eating. 



February 14, died, presenting typical symptoms and lesions 

 of rinderpest. 



