130 The Philippine Journal of Science ms 



EXPERIMENT 2 



Water extract of liver, spleen, and lymph glands, 3 days old. 



Carabao 72. — Known history prior to the experiment : Native 

 Fuga carabao, 2 years old, received at the laboratory and placed 

 in quarantine January 24, 1917. This animal was kept under 

 observation for twelve days before it was used and at no time 

 during this period did it present a high temperature or develop 

 any symptoms of sickness. 



February 6, 1917, carabao 72 was injected subcutaneously 

 with 100 cubic centimeters of the same extract as was used in 

 experiment 1. 



February 9, carabao 72 presented an afternoon temperature 

 of 40.5° C. 



February 10-12, diarrhoea, not eating. 



February 13, found dead in the morning. This animal pre- 

 sented typical symptoms and lesions of rinderpest. 



From the results of these two experiments it will be noticed 

 that the watery extract from the organs used was very potent, 

 the incubation period being four and three days, respectively, 

 and the various symptoms leading up to death were prompt in 

 making their appearance after the initial rise in temperature. 



EXPERIMENT 3 



Phenol (0.5 per cent) extract of liver and lymph glands, 5 

 days old. 



Carabao 75. — Known history prior to the experiment: Native 

 Fuga carabao, 2 years old, received at the laboratory and placed 

 in quarantine January 24, 1917. This animal was kept under 

 observation for twenty-two days before it was used, and at no 

 time during this period did it present a high temperature or 

 develop any symptoms of sickness. 



February 16, 1917, carabao 75 was injected subcutaneously 

 with 200 cubic centimeters of a 4-day-old 0.5 per cent phenol ex- 

 tract from the liver and lymph glands of carabao 73, which was 

 bled to death on the second day of temperature for virulent blood, 

 to be used in immunization work. 



The extract was prepared as follows : 



Liver, 250 grams ; 0.5 per cent phenol, 500 cubic centimeters. 



Lymph glands, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 



This was placed in the refrigerator, filtered through gauze on 

 the third day, and returned to the refrigerator. The material 

 had a sweet odor, presenting no evidence of putrefaction. 



February 20, carabao 75 presented an afternoon temperature 

 of 39.5° C. 



