xiii, b, 3 Boynton: Virulence of Body Organs in Rinderpest 131 



February 21, morning temperature, 39° C. 

 February 22-23, diarrhoea, not eating. 



February 24, found dead in the morning. This animal pre- 

 sented typical symptoms and lesions of rinderpest. 



EXPERIMENT 4 



Phenol (0.5 per cent) extract of liver, spleen, and lymph glands, 

 5 days old. 



Carabao 81. — Known history prior to the experiment: Native 

 Fuga carabao, 2 years old, received at the laboratory and placed 

 in quarantine January 24, 1917. This animal was kept under 

 observation for thirty-four days before it was used, and at no 

 time during this period did it present a high temperature or 

 develop any symptoms of sickness. 



February 28, 1917, carabao 81 was injected subcutaneously 

 with 200 cubic centimeters of a 5-day-old 0.5 per cent phenol 

 extract of the liver, spleen, and lymph glands from carabao 77, 

 which was bled to death on the second day of temperature for 

 virulent blood, to be used in immunization work. 



The extract was prepared as follows : 



Liver, 250 grams; 0.5 per cent phenol, 500 cubic centimeters. 

 Spleen, 175 grams; 0.5 per cent phenol, 350 cubic centimeters. 

 Lymph glands, 135 grams; 0.5 per cent phenol, 270 cubic centimeters. 



This was placed in the refrigerator for three days ; it was then 

 filtered through gauze, and the filtrate was returned to the re- 

 frigerator. When the extract was injected, it had a sweet odor, 

 presenting no evidence of putrefaction. 



March 2, carabao 81 presented a forenoon temperature of 39.5° 

 C. and an afternoon temperature of 40° C. 



March 4-7, diarrhoea, not eating. 



March 8, found dead in the morning. This animal presented 

 typical symptoms and lesions of rinderpest. 



EXPERIMENT 5 



Phenol (0.5 per cent) extract of heart, 5 days old. 



Carabao 8U- — Known history prior to the experiment : Native 

 Fuga carabao, 3 years and 2 months old, received at the labo- 

 ratory and placed in quarantine January 24, 1917. This animal 

 was kept under observation for fifty-six days before it was used 

 in this experiment. On March 8, 1917, it was injected with 10 

 cubic centimeters of culture material in which the virus of rinder- 

 pest had been inoculated. The animal presented no ill effects 

 from this injection. 



March 22, 1917, carabao 84 was injected subcutaneously with 



