132 The Philippine Journal of Science me 



200 cubic centimeters of a 5-day-old 0.5 per cent phenol extract 

 of the heart from carabao 85, which was bled to death on the 

 fourth day of temperature for virulent blood, to be used in im- 

 munization work. 



The extract was prepared as follows: 



Heart, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 



This was placed in the refrigerator for three days; it was 

 then filtered through gauze, and the nitrate was returned to the 

 refrigerator. When this extract was used, it had a sweet odor 

 and presented no signs of putrefaction. 



March 28, carabao 84 presented a forenoon temperature of 

 39.6° C. and an afternoon temperature of 40.7° C. 



March 29, diarrhoea. 



March 30, diarrhoea, eating little. 



March 31, this animal was bled to death for virulent blood, 

 to be used in immunization work. It presented good lesions of 

 rinderpest. 



EXPERIMENT G 



Phenol (0.5 per cent) extract of skeletal muscle, 5 days old. 



Carabao 88. — Known history prior to the experiment: Jolo 

 carabao, 8 years and 4 months old, received at the laboratory 

 and placed in quarantine March 3, 1917. This animal was kept 

 under observation for twenty-six days before it was used, and 

 at no time during this period did it present a high temperature 

 or develop any symptoms of sickness. 



March 29, 1917, carabao 88 was injected subcutaneously with 

 200 cubic centimeters of a 5-day-old 0.5 per cent phenol extract 

 of skeletal muscle from carabao 263 (P. C. W.), which was bled 

 to death on the first day of temperature for virulent blood, to 

 be used for hyperimmunization work in the production of anti- 

 rinderpest serum. 



The extract was prepared as follows : 



Skeletal muscle, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 



This was placed in the refrigerator for three days; it was 

 then filtered through gauze, and the filtrate was returned to the 

 refrigerator. 



When the extract was injected, it had a sweet odor, present- 

 ing no evidence of putrefaction. 



Carabao 88 did not developed any ill effects from this injection. 



April 15, 1917, which was seventeen days after the muscle- 

 extract injection, this animal was injected subcutaneously with 



