xiii, b, 3 Boynton: Virulence of Body Organs in Rinderpest 133 



50 cubic centimeters of virulent blood, to test its susceptibility 

 to rinderpest. 



April 19, this animal presented an afternoon temperature of 

 40.7° C. 



April 21-22, diarrhoea, eating little. 



April 23-25, diarrhoea, not eating; blood and mucous casts in 

 the fseces. 



April 26, found dead in the morning. This animal presented 

 typical symptoms and lesions of rinderpest, which proves that 

 it was highly susceptible to that disease when it was injected 

 with the muscle extract. This result also leaves the impression 

 that the virus of rinderpest is not harbored in the skeletal mus- 

 cles or that it is easily destroyed in this tissue. 



EXPERIMENT 7 



Phenol (0.5 per cent) extract of liver, spleen, and lymph 

 glands, 15 days old. 



Carabao 93. — Known history prior to the experiment: Jolo 

 carabao, 8 years and 4 months old, received at the laboratory 

 and placed in quarantine March 3, 1917. This animal was kept 

 under observation for fifty-two days before it was used, and at 

 no time during this period did it present a high temperature or 

 develop any symptoms of sickness. 



April 25, 1917, carabao 93 was injected subcutaneously with 

 200 cubic centimeters of a 15-day-old 0.5 per cent phenol extract 

 of liver, spleen, and lymph glands from carabao 251 (P. C. W.), 

 which was bled to death on the second day of temperature for 

 virulent blood to be used for hyperimmunization work in the 

 production of antirinderpest serum. 



The extract was prepared as follows : 



Liver, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 

 Spleen, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 

 Lymph glands, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 



This was placed in the refrigerator for three days ; it was then 

 filtered through gauze, and the filtrate was returned to the re- 

 frigerator. When the extract was injected, it had a sweet odor, 

 presenting no evidence of putrefaction. 



April 29, carabao 93 presented a forenoon temperature of 39.2° 

 C. and an afternoon temperature 40.2° C. 



April 30, diarrhoea; afternoon temperature, 40.9° C. 



May 1, diarrhoea, eating little. 



May 2-3, diarrhoea, not eating. 



May 4, found dead in the morning. This animal presented 

 typical symptoms and lesions of rinderpest. 



