134 The Philippine Journal of Science 1918 



EXPERIMENT 8 



Phenol (0.5 per cent) extract of caecum and of colon, 5 days old. 



Bull 4264. — Known history prior to the experiment: Native 

 Fuga bull, 4 years old, received at the laboratory and placed 

 in quarantine April 12, 1917. This animal was kept under ob- 

 servation for three days before it was used; it did not present 

 a high temperature or develop any symptoms of sickness during 

 this time. 



April 15, 1917, bull 4264 was injected subcutaneously with 

 100 cubic centimeters of a 5-day-old 0.5 per cent phenol extract 

 of the caecum and colon from carabao 251 (P. C. W.), mentioned 

 in experiment 7. 



The extract was prepared as follows : 



Caecum and colon, 200 grains; 0.5 per cent phenol, 400 cubic centi- 

 meters. 



This was placed in the refrigerator for three days ; it was then 

 filtered through gauze, and the filtrate was returned to the re- 

 frigerator. When the extract was injected, it had a sweet odor, 

 presenting no evidence of putrefaction. 



April 20, bull 4264 presented an afternoon temperature of 

 40.9° C. 



April 21, afternoon temperature, 41.1° C. 



April 22-23, diarrhoea, not eating. 



April 24, bled to death for virulent blood, to be used in im- 

 munization work. This animal presented typical symptoms and 

 lesions of rinderpest. 



EXPERIMENT !) 



Phenol (0.5 per cent) extract of larynx, pharynx, and base of 

 tongue, 5 days old. 



Bull 4265. — Known history prior to the experiment: Native 

 Fuga bull, 4 years old, received at the laboratory and placed 

 in quarantine April 12, 1917. This animal was kept under 

 observation for seven days before it was used ; it did not present 

 a high temperature or develop any symptoms of sickness during 

 this period. 



April 20, 1917, bull 4265 was injected subcutaneously with 

 100 cubic centimeters of a 5-day-old 0.5 per cent phenol extract 

 of the larynx, pharynx, and base of tongue from carabao 240 

 (P. C. W.), which was bled to death on the third day of tem- 

 perature for virulent blood to be used in immunization. 



The extract was prepared as follows : 



