xiu, b, 3 Boynton: Virulence of Body Organs in Rinderpest 135 



Larynx, 50 grams; 0.5 per cent phenol, 100 cubic centimeters. 

 Pharynx, 50 grams; 0.5 per cent phenol, 100 cubic centimeters. 

 Base of tongue, 50 grams; 0.5 per cent phenol, 100 cubic centimeters. 



This was placed in the refrigerator for three days; it was 

 then filtered through gauze, and the filtrate was returned to the 

 refrigerator. 



When the extract was injected, it had a sweet odor, presenting 

 no evidence of putrefaction. 



Bull 4265 did not develop any ill effects from this injection. 



May 7, 1917, which was seventeen days after the injection 

 of the above-mentioned extracts, this animal was injected sub- 

 cutaneously with 50 cubic centimeters of virulent blood. 



May 10, bull 4265 presented its first rise in temperature, 

 registering, in the forenoon, 39° C; in the afternoon, 40° C. 



May 11, forenoon temperature, 39.9° C. ; bled to death for 

 virulent blood, to be used in immunization work. This animal 

 presented lesions found in the early stages of rinderpest. This 

 proves that bull 4265 was susceptible to the disease when it re- 

 ceived the injection of extracts. It also leads to the idea that 

 the virus from these parts is either scarce or is easily destroyed 

 by the above method of handling, as it will be noticed in ex- 

 periment 10 that other tissues from the same animal were virulent 

 eight days after extraction. 



EXPERIMENT 10 



Phenol (0.5 per cent) extract of liver, spleen, and lymph 

 glands, 8 days old. 



Bull 4266. — Known history prior to the experiment: Native 

 Fuga bull, 3 years old, received at the laboratory and placed 

 in quarantine April 12, 1917. This animal was kept under ob- 

 servation for ten days before it was used, and it did not present a 

 high temperature or develop any symptoms of sickness during 

 this period. 



April 23, 1917, bull 4266 was injected subcutaneously with 

 50 cubic centimeters of an 8-day-old 0.5 per cent phenol extract 

 of the liver, spleen, and lymph glands from carabao 240 (P. C. 

 W.), mentioned in experiment 9. 



The extract was prepared as follows: 



Liver, 150 grams; 0.5 per cent phenol, 300 cubic centimeters. 

 Spleen, 150 grams; 0.5 per cent phenol, 300 cubic centimeters. 

 Lymph glands, 100 grams ; 0.5 per cent phenol, 200 cubic centimeters. 



This was placed in the refrigerator for three days; it was 



