136 The Philippine Journal of Science ms 



then filtered through gauze, and the filtrate was returned to the 

 refrigerator. 



When the extract was injected, it had a sweet odor, presenting 

 no evidence of putrefaction. 



April 26, bull 4266 presented its first rise in temperature, 

 registering, in the forenoon, 38.9° C. ; in the afternoon, 40.5° C. 



April 28-30 diarrhoea, not eating. 



May 1-2, diarrhoea, not eating. 



May 3, found dead in the morning. This animal presented 

 typical symptoms and lesions of rinderpest. 



EXPERIMENT 11 



Phenol (0.5 per cent) extract of liver, spleen, and lymph 

 glands, 15 days old. 



Three animals were used in this experiment, bulls 4269, 4270, 

 and 4271. All these animals received a similar amount of the 

 same extract on the same day, and all gave similar results. 

 Therefore one only, bull 4269, will be considered. 



Bull 4269. — Known history prior to the experiment: Native 

 Fuga bull, 3 years old, received at the laboratory and placed in 

 quarantine April 12, 1917. This animal was kept under ob- 

 servation for twelve days before it was used; it did not present 

 a high temperature or develop any symptoms of sickness during 

 this period. 



April 25, 1917, bull 4269 was injected subcutaneously with 

 200 cubic centimeters of a 15-day-old 0.5 per cent phenol extract 

 of the liver, spleen, and lymph glands from carabao 251 (P. C. 

 W.), which was bled to death on the second day of temperature 

 for virulent blood, to be used in immunization work. 



The extract was prepared as follows : 



Liver, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 

 Spleen, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 

 Lymph glands, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 



This was placed in the refrigerator for three days ; it was then 

 filtered through gauze, and the filtrate was returned to the re- 

 frigerator. The liver extract had a slight butyric acid odor when 

 it was injected ; the others were sweet. 



April 27, bull 4269 presented its first rise in temperature, 

 registering, in the forenoon, 39.1° C; in the afternoon, 40.3° C. 



April 28, forenoon temperature, 40° C. ; afternoon temperature, 

 41.2° C. 



April 30, diarrhoea, eating little. 



May 1, forenoon temperature, 40.5° C. ; bled to death for 

 virulent blood, to be used in immunization work. This ani- 



