xni, b, 3 Boynton: Virulence of Body Organs in Rinderpest 137 



mal presented typical symptoms and lesions of a severe case of 

 rinderpest. 



Bulls 4270 and 4271 died of rinderpest during the forenoon 

 of May 4. Both animals presented typical symptoms and lesions 

 of rinderpest. 



EXPERIMENT 12 



Phenol (0.5 per cent) extract of liver, spleen, and parotid 

 and lymph glands, 16 days old. 



Cow 4260. — Known history prior to the experiment: Native 

 Fuga cow, 2 years old, received at the laboratory and placed 

 in quarantine April 12, 1917. This animal was kept under 

 observation for twenty-one days before it was used; it did not 

 present a high temperature or develop any symptoms of sickness 

 during this period. 



May 4, 1917, cow 4260 was injected subcutaneously with 200 

 cubic centimeters of a 16-day-old 0.5 per cent phenol extract of 

 the liver, spleen, and parotid and lymph glands from carabao 

 228 (P. C. W.), which was bled to death on the first day of 

 temperature for virulent blood, to be used in immunization work. 



The extract was prepared as follows : 



Liver, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 

 Spleen, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 

 Parotid, 100 grams; 0.5 per cent phenol, 200 cubic centimeters. 

 Lymph glands, 150 grams; 0.5 per cent phenol, 300 cubic centimeters. 



This was placed in the refrigerator for three days ; it was then 

 filtered through gauze, and the filtrate was returned to the re- 

 frigerator. When the extract was injected, it had a sweet odor, 

 presenting no evidence of putrefaction. 



May 7, cow 4260 presented its first rise in temperature, reg- 

 istering, in the afternoon, 40.3° C. 



May 10, not eating. 



May 11-12, diarrhoea, not eating. 



May 13, found dead in the morning. This animal presented 

 typical symptoms and lesions of rinderpest. 



EXPERIMENT 13 



Phenol (0.5 per cent) extract of liver, spleen, and parotid 

 and lymph glands, 20 days old. 



Bull 4272. — Known history prior to the experiment: Native 

 Fuga bull, 2 years old, received at the laboratory and placed in 

 quarantine April 12, 1917. This animal was kept under obser- 

 vation twenty-seven days before it was used; it did not present 

 a high temperature or develop any symptoms of sickness during 

 this period. 



