138 The Philippine Journal of Science ms 



May 10, 1917, bull 4272 was injected subcutaneously with 

 200 cubic centimeters of a 20-day-old 0.5 per cent phenol extract 

 of the liver, spleen, and parotid and lymph glands from carabao 

 228 (P. C. W.), the preparation of which is described in experi- 

 ment 12. The liver extract had a slight butyric acid odor at 

 the time of injection. The other extracts had a sweet odor and 

 presented no evidence of putrefaction. 



May 14, bull 4272 presented its first rise in temperature, reg- 

 istering, in the forenoon, 39.4° C. ; in the afternoon, 40.4° C. 



May 16-18, diarrhoea, eating little. 



May 19-20, diarrhoea, not eating. 



May 21, animal died early in the forenoon, presenting typical 

 symptoms and lesions of rinderpest. 



EXPERIMENT 14 



Phenol (0.5 per cent) extract of liver, spleen, and lymph 

 glands, 15 days old. 



Carabao 92. — Known history prior to the experiment : Native 

 Jolo carabao, 3 years and 6 months old, received at the labora- 

 tory and placed in quarantine March 3, 1917. This animal was 

 kept under observation for fifty -two days before it was used; 

 it did not present a high temperature or develop any symptoms 

 of sickness during this period. 



April 25, 1917, carabao 92 was injected subcutaneously with 

 200 cubic centimeters of a 15-day-old 0.5 per cent phenol extract 

 of the liver, spleen, and lymph glands from carabao 251 (P. C. 

 W.), which was bled to death on the second day of temperature 

 for virulent blood, to be used in immunization work. 



The extract was prepared as follows : 



Liver, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 

 Spleen, 200 grams; 0.5 per cent phenol, 400 cubic centimeters. 

 Lymph glands, 150 grams; 0.5 per cent phenol, 300 cubic centimeters. 



This was placed in the refrigerator for three days; it was 

 then filtered through gauze, and the filtrate was returned to the 

 refrigerator. 



When the extract was injected, it had a sweet odor, present- 

 ing no evidence of putrefaction. 



April 28, this animal presented its first rise in temperature, 

 registering, in the forenoon, 39.4° C. ; in the afternoon, 39.6° C. 



May 1, diarrhoea; forenoon temperature, 39.8° C. ; afternoon 

 temperature, 41.1° C. 



May 2—3, diarrhoea, not eating. 



May 4, diarrhoea, eating little. 



