140 The Philippine Journal of Science ms 



was markedly attenuated, having just enough vitality to cause a 

 slight onset of the disease, which led to a speedy recovery. 



EXPERIMENT 16 



Phenol (0.5 per cent) extract of pancreas, 5 days old. 



Carabao 107. — Known history prior to the experiment: Native 

 Jolo carabao, 5 years and 6 months old, received at the labora- 

 tory and placed in quarantine March 3, 1917. This animal was 

 kept under observation eighty days before it was used and did 

 not present a high temperature or develop any symptoms of 

 sickness during that period. 



May 23, 1917, carabao 107 received subcutaneously 100 cubic 

 centimeters of a 5-day-old 0.5 per cent phenol extract of pancreas 

 from bull 4 (P. C. W.), which was bled to death on the first day 

 of temperature for virulent blood, to be used in serum production. 



The extract was prepared as follows : 



Pancreas, 100 grams; 0.5 per cent phenol, 200 cubic centimeters. 



This was placed in the refrigerator for three days; it was 

 then filtered through gauze, and the filtrate was returned to the 

 refrigerator. 



Carabao 107 never developed any ill effects from this injection. 



June 13, which was twenty-one days after the pancreas-extract 

 injection, carabao 107 received 50 cubic centimeters of virulent 

 blood. 



June 18, carabao 107 presented its first rise in temperature, 

 registering, in the forenoon, 39.8° C; in the afternoon, 40.6° C. 



June 19, diarrhoea. 



June 20-21, diarrhoea, not eating. 



June 22, found dead in the morning. This animal presented 

 typical symptoms and lesions of rinderpest. 



From this result it appears that the pancreas extracted in this 

 manner is not virulent after five days. 



EXPERIMENT 17 



Phenol (0.5 per cent) extract of liver, spleen, and parotid and 

 lymph glands, 55 days old. 



Bull 4285. — Known history prior to the experiment: Native 

 Fuga bull, 3 years old, received at the laboratory and placed in 

 quarantine May 8, 1917. This animal was kept under observa- 

 tion thirty-four days before it was used; it did not present a 

 high temperature or develop any symptoms of sickness during 

 this period. 



June 12, 1917, bull 4285 received subcutaneously 120 cubic 

 centimeters of a 55-day-old 0.5 per cent phenol extract of liver, 



