xiii, b, 3 Boynton: Virulence of Body Organs in Rinderpest 141 



spleen, and parotid and lymph glands from carabao 228 (P. C. 

 W.). This extract was from the same lot used in experiment 

 12. At this time the liver extract had a slight butyric acid odor. 



June 20, bull 4285 presented its first rise in temperature, reg- 

 istering, in the forenoon, 39° C. ; in the afternoon, 40.5° C. 



June 21, diarrhoea; forenoon temperature, 40° C; afternoon, 

 40.6° C. 



June 22-24, diarrhoea, not eating. 



June 25, found dead in the morning. This animal presented 

 typical symptoms and lesions of rinderpest. 



From this result it appears possible to keep the virus in a 

 virulent form for as long a period as fifty-five days in a 0.5 per 

 cent phenol solution. 



EXPERIMENT 18 



Phenol (1 per cent) extract of lymph glands, 6 days old. 



Bull 4-296. — Known history prior to experiment: Native Fuga 

 bull, 2 years old, received at the laboratory and placed in quaran- 

 tine June 1, 1917. This animal was kept under observation for 

 seventeen days before it was used; it did not present a high 

 temperature or develop any symptoms of sickness during this 

 period. 



June 18, 1917, bull 4296 received subcutaneously 100 cubic 

 centimeters of a 6-day-old 1 per cent phenol extract of lymph 

 glands from bulls 1036 and 1037 (P. C. W.), which were bled to 

 death on the second day of temperature for virulent blood, to be 

 used in the production of antirinderpest serum. 



The extract was prepared as follows: 



Lymph glands, 200 grams; 1 per cent phenol, 400 cubic centimeters. 



This was placed in the refrigerator for three days; it was 

 then filtered through gauze, and the filtrate was replaced in the 

 refrigerator. 



June 22, bull 4296 presented its first rise in temperature, 

 registering, in the forenoon, 39.2° C. ; in the afternoon, 41.° C. 



June 24-27, diarrhoea, not eating. 



June 28, died during the forenoon. This animal presented 

 typical symptoms and lesions of rinderpest, which proves that a 

 1 per cent phenol solution will not destroy the virus of rinderpest 

 in the lymph glands over a period of six days, nor does the virus 

 appear to be attenuated by its presence. 



EXPERIMENT 19 



Phenol (1 per cent) extract of liver, spleen, caecum, and lymph 

 glands, 17 days old. 



