142 The Philippine Journal of Science i»*» 



Bull 4299. — Known history prior to experiment : Native Fuga 

 bull, 2 years old, received at the laboratory and placed in quar- 

 antine June, 1, 1917. This animal was kept under observation 

 for twenty-five days before it was used ; it did not present a high 

 temperature or develop any symptoms of sickness during this 

 period. 



The extract to be used was prepared as follows : 



Liver, 100 grams; 1 per cent phenol, 200 cubic centimeters. 

 Spleen, 100 grams; 1 per cent phenol, 200 cubic centimeters. 

 Caecum, 100 grams; 1 per cent phenol, 200 cubic centimeters. 

 Lymph glands, 100 grams ; 1 per cent phenol, 200 cubic centimeters. 



This was placed in the refrigerator for three days; it was 

 then filtered through gauze, and the filtrate was returned to the 

 refrigerator. 



June 25, 50 cubic centimeters of each extract were added, 

 making a total of 200 cubic centimeters; to this mixed extract 

 20 cubic centimeters of a 1-1,000 per cent chlorazene solution 

 were added, and the resulting mixture was returned to the re- 

 frigerator. 



June 26, 1917, bull 4299 received subcutaneously 100 cubic 

 centimeters of the 17-day-old 1 per cent phenol extract of liver, 

 spleen, lymph glands, and csecum from bull 1034 (P. C. W.), 

 which was bled to death on the first day of temperature for 

 virulent blood, to which the 1-1,000 per cent chlorazene solution 

 had been added on the previous day. 



July 1, 1917, bull 4299 presented its first rise in temperature, 

 registering, in the forenoon, 39.9° C; in the afternoon, 39.8° C. 



July 2, forenoon temperature, 40.1° C. ; afternoon, 40.2° C. 



July 3-5, diarrhcea. 



July 6, died in the forenoon, presenting good symptoms and 

 lesions of rinderpest. 



This shows that the 1 per cent phenol solution after acting 

 seventeen days and the 1-1,000 per cent chlorazene solution 

 after acting one day upon the mixed tissue extracts did not have 

 any apparent detrimental effect upon the virus of rinderpest. 



EXPERIMENT L'li 



Phenol (1 per cent) extract of lymph glands, 20 days old. 



Bull 4302. — Known history prior to experiment : Native Fuga 

 bull, 2 years old, received at the laboratory and placed in quar- 

 antine June 1, 1917. This animal was kept under observation 

 for twenty-eight days before it was used; it did not present 

 a high temperature or develop any symptoms of sickness during 

 this period. 



