144 The Philippine Journal of Science ms 



July 3, this animal presented its initial rise in temperature, 

 registering, in the forenoon, 39.4° C; in the afternoon, 40.6° C. 



July 6-8, diarrhoea, not eating. 



July 9, died, presenting good symptoms and lesions of rin- 

 derpest. 



It will be noticed from the results obtained in experiments 

 20, 21, and 22 that the 1 per cent phenol had apparently no 

 detrimental effect upon the virus of rinderpest when contained 

 in the lymph glands for twenty days and in the liver and in the 

 spleen for twenty-one days. 



EXPERIMENT 23 



Phenol (1 per cent) extract of lymph glands, 17 days old. 



Bull 4306. — Known history prior to experiment : Native Fuga 

 bull, 3 years old, received at the laboratory and placed in quar- 

 antine June 1, 1917. This animal was kept under observation 

 for thirty-eight days before it was used; it did not present a 

 high temperature or develop any symptoms of sickness during 

 this period. 



July 9, 1917, bull 4306 received subcutaneously 100 cubic 

 centimeters of a 17-day-old 1 per cent phenol extract of lymph 

 glands from carabao 107, which died of rinderpest on the sixth 

 day of temperature (see experiment 16). 



The extract was prepared as follows: 



Lymph glands, 200 grams; 1 per cent phenol, 400 cubic centimeters. 



This was placed in the refrigerator for three days; it was 

 then filtered through gauze and returned to the refrigerator. 



This animal ran an atypical course of the disease. On July 

 10, the afternoon temperature was 39.4° C, which was the 

 highest temperature registered. 



July 13-14, diarrhoea, not eating. 



July 15, found dead in the morning. This animal presented 

 good physical symptoms and good lesions of rinderpest, but did 

 not develop a high temperature. The disease was very acute, 

 as the animal was dead on the morning of the sixth day after 

 injection. 



EXPERIMENT 24 



Phenol (2 per cent) extract of spleen, 5 days old. 



Bull 4316. — Known history prior to experiment: Native Ba- 

 tanes bull, 6 years old, received at the laboratory and placed in 

 quarantine June 3, 1917. This animal was kept under observa- 

 tion for seventy -two days before it was used ; it did not present 

 a high temperature or develop any symptoms of sickness during 

 this period. 



