xiii, b, 3 Boynton: Virulence of Body Organs in Rinderpest 145 



August 15, 1917, bull 4316 received subcutaneously 50 cubic 

 centimeters of a 5-day-old 2 per cent phenol extract of spleen 

 from a bull (P. C. W.) that was bled to death for virulent blood 

 on its second day of temperature. This spleen extract was 

 agitated at room temperature for forty-eight hours. 



The extract was prepared as follows: 



Spleen, 100 grams; 2 per cent phenol, 200 cubic centimeters. 



This was placed in a shaking machine and agitated contin- 

 uously for forty-eight hours at room temperature. At the ex- 

 piration of forty-eight hours it was placed in the refrigerator 

 for twenty-four hours; it was then filtered through gauze, and 

 the filtrate was returned to the refrigerator. 



August 20, afternoon temperature, 39.8° C. 



August 21, forenoon temperature, 38.7° C; afternoon, 39.8° C. 



August 22, forenoon temperature, 39° C. ; afternoon, 40.2° C. 



August 25-26, eating little. 



This animal was given 600 cubic centimeters of antirinderpest 

 serum on August 21, 200 cubic centimeters on August 22, and 

 100 cubic centimeters on August 25. With the mildness of the 

 attack and the administration of the serum the animal made a 

 speedy recovery. 



This animal was constantly exposed to animals sick with 

 rinderpest. On September 13, 1917, it was injected with 2,000 

 cubic centimeters of a 7-day-old 0.75 per cent phenol extract of 

 liver and lymph glands. It never developed the disease, proving 

 that it had been immunized by its first attack. 



September 27, 1917, it was considered hyperimmune and was 

 bled to death for its serum. 



This proves that the 2 per cent phenol and the agitation did 

 not destroy the virus of rinderpest, but undoubtedly attenuated 

 it to some extent. 



EXPERIMENT 25 



Phenol (2 per cent) and glycerin extract of spleen, 5 days old. 



Bull 4308. — Known history prior to experiment: Native Fuga 

 bull, 3 years and 3 months old, received at the laboratory and 

 placed in quarantine June 1, 1917. This animal was kept under 

 observation seventy-four days before it was used; it did not 

 present a high temperature or develop any symptoms of sickness 

 during this period. 



August 15, 1917, bull 4308 received subcutaneously 50 cubic 

 centimeters of a 5-day-old 2 per cent phenol extract of spleen, 

 to which glycerin was added, from the bull (P. C. W.) mentioned 

 in experiment 24. 



