146 The Philippine Journal of Science ms 



The extract was prepared as follows : 



Spleen, 100 grams. 

 Glycerin, 50 cubic centimeters. 

 Water, 150 cubic centimeters. 

 Phenol (pure), 4 cubic centimeters. 



This was placed in a shaking machine and agitated con- 

 tinuously for forty-eight hours at room temperature. At the 

 expiration of forty-eight hours it was placed in the refrigerator 

 for twenty -four hours; it was then filtered through gauze, and 

 the filtrate was returned to the refrigerator. 



Bull 4308 did not develop any symptoms of disease from this 

 injection. 



August 29, which was fourteen days after the extract injec- 

 tion, this animal was injected with 50 cubic centimeters of 

 virulent blood. 



September 1, this animal presented its first rise in temper- 

 ature, registering, in the forenoon, 39.5° C; in the afternoon, 

 40.1° C. 



It ran a rather severe course of the disease, but recovered. 



This proves that the virus was destroyed in the spleen extract 

 by the action of the 2 per cent phenol and glycerin. 



Several similar experiments were tried, using liver and lymph 

 glands, and it was found that the virus was destroyed in each 

 case where glycerin was added. 



EXPERIMENT 26 



Phenol (2 per cent) extract of lymph glands, 8 days old. 



Bull 4335. — Known history prior to experiment : Native Fuga 

 bull, 3 years old, received at the laboratory and placed in quaran- 

 tine August 28, 1917. This animal was used on the day of its 

 arrival; consequently it was not under observation previous to 

 the experiment. 



August 28, 1917, bull 4335 received subcutaneously 50 cubic 

 centimeters of an 8-day-old 2 per cent phenol extract of lymph 

 glands from bull 1660 (P. C. W.), which was bled to death on 

 its second day of temperature for virulent blood. 



The extract was prepared as follows : 



Lymph glands, 100 grams; 2 per cent phenol, 200 cubic centimeters. 



This was placed in the shaking machine and agitated con- 

 tinuously for forty-eight hours at room temperature. At the 

 expiration of forty-eight hours it was placed in the refrigerator 

 for twenty-four hours; it was then filtered through gauze, and 

 the filtrate was returned to the refrigerator. 



This injection had no ill effect upon bull 4335. 



