xiii, b, 3 Boynton: Virulence of Body Organs in Rinderpest 147 



September 11, 1917, which was fourteen days after the 2 per 

 cent phenol and lymph gland extract injection, this animal re- 

 ceived 50 cubic centimeters of a 5-day-old 0.75 per cent phenol 

 extract of liver and lymph glands from carabao 97, which was 

 bled to death for virulent blood on its fourth day of temperature. 



September 13, bull 4335 presented its first rise in temperature, 

 registering, in the forenoon, 39.6° C. ; in the afternoon, 39.8° C. 



September 14, forenoon temperature, 40.2° C. ; afternoon, 

 40.3° C. 



September 15, diarrhoea; bled to death for virulent blood, to 

 be used in immunization work. This animal presented good 

 symptoms and lesions of rinderpest. 



This proves that the 2 per cent phenol and the agitation 

 together destroyed the virus of rinderpest in the lymph gland 

 extract after eight days. 



Similar results were obtained by treating liver tissue in the 

 same way and for the same length of time. 



Heart and intestine extracts were found to lose their virulence 

 in six days when treated in the above-mentioned manner. 



DISCUSSION 



From the results obtained in the foregoing experiments, it is 

 apparent that the virus of rinderpest held in certain tissues of 

 the body is not injured when extracted with weak solutions of 

 phenol. From many observations that have been made in this 

 laboratory during the past seven years, it has been noticed that 

 the virus of rinderpest is quickly destroyed in decomposing 

 material, either tissue or blood. On the other hand, if virulent 

 blood is drawn under aseptic conditions and placed in sterile 

 containers, the virus will retain its activity for five or six days. 

 If the blood is kept in a clotted form, the virus retains its activity 

 a few days longer. It has been shown in previous work (2) 

 that when the large water leech (Hirudo boyntoni Wharton) 

 is allowed to feed upon an animal sick with rinderpest, the virus 

 may remain active for a period of twenty-five days inside the 

 body of the leech. In this case the blood is kept from putre- 

 factive organisms and also in a semianaerobic condition. 



By extracting certain organs with weak phenol solutions, the 

 activity of the putrefactive organisms is kept down to the mini- 

 mum, and thus they have little or no effect upon the virus of 

 rinderpest. 



Many times a certain method will work in the hands of the 

 originator, but when placed in other hands the same good results 

 are not obtained. To check this, Drs. Ildefonso Patdu and 



