148 The Philippine Journal of Science ms 



Florencio Patenia have made these extracts with no supervision 

 from me, and the results obtained from these extracts were 

 similar to those obtained where I had had full supervision. 



To prove that these extracts would work as readily upon 

 animals not used in the laboratory, on three different occasions 

 extracts were given to Dr. D. W. Shaffer and Mr. Thomas L. 

 Bean to be used as virulent material on animals that were used 

 in the production of antirinderpest serum in the Philip C. 

 Whitaker antirinderpest serum laboratory. Doctor Shaffer and 

 Mr. Bean obtained as good results with the extracts as we had 

 in the research laboratory, which proves that these extracts 

 work as readily on animals outside as on those inside of the 

 laboratory. 



These extracts have been used in the immunization stations 

 in the provinces, under the supervision of Dr. Stanton Young- 

 berg, chief veterinarian. 2 In preparing extracts for the prov- 

 inces, we use a 0.75 per cent phenol solution. For ordinary 

 immunization work it is best not to use an extract over 15 days 

 old, as there are other factors that enter in that are apt to 

 delude. We have obtained a considerable number of very grat- 

 ifying results with old extracts, which will be reported in a 

 subsequent paper. On these occasions the animals presented 

 no reaction to the injection. After a period of two weeks these 

 animals were exposed to rinderpest by various methods, that is, 

 by exposure to sick animals, inoculation with virulent blood, and 

 inoculation with extracts. These animals presented no ill effects 

 from the exposures to which they were subjected, showing that 

 they had been immunized by the primary injection of extract. 



From the result obtained by Birch on hog cholera, (l) it is 

 possible that tissue extracts can be used as readily in that disease 

 as in rinderpest, thereby lowering the enormous expense of ob- 

 taining virulent material in the production of antihog-cholera 

 serum. 



I wish to thank Dr. D. W. Shaffer for the privilege of securing 

 various tissues used in these experiments from animals used by 

 him in obtaining virulent blood in the process of making anti- 

 rinderpest serum. 



CONCLUSIONS 



1. From the results obtained in experiments 1 and 2, it is 

 evident that water extracts of the liver, spleen, and lymph glands, 

 3 days old, are highly infectious to susceptible animals. 



2. From the results obtained in experiments 3 and 4, it is 



"-Phil. Agr. Rev. (1917), 10. 



