154 The Philippine Journal of Science ms 



Doctor Youngberg had the extracts used in simultaneous im- 

 munization of carabao at the immunization station at Lubao, 

 Pampanga Province, Doctor Topacio doing the work. These 

 extracts were tried on two different sets of animals. 



July 22, 1917, seventeen head of carabao were brought to the 

 station for immunization and were injected with mixed liver, 

 spleen, and lymph gland extract in a 0.5 per cent phenol solution, 

 5 days old. The doses administered and the final results were 

 as follows: Eight animals received 5 cubic centimeters each of 

 this extract, five animals giving good reactions; four animals 

 received 10 cubic centimeters each of the extract, all of them 

 giving good reactions; two animals received 15 cubic centimeters 

 each of the extract, neither reacting; two animals received 

 20 cubic centimeters each of the extract, one reacting; one 

 animal received 25 cubic centimeters of the extract without 

 reacting. On August 5, 1917, all the animals that did not react 

 were injected with 25 cubic centimeters of virulent blood, and 

 none of them developed the disease, proving them to be immune. 

 When these animals were injected with the extract, they also 

 received from 250 to 600 cubic centimeters of antirinderpest 

 serum, the amount of serum administered depending upon the 

 size of the animal. 



August 17, 1917, fifteen head of carabao, brought into the 

 station for immunization, received 5 cubic centimeters each of 

 an 8-day-old liver extract that had been prepared as follows : 

 Two hundred grams of liver from an animal bled to death on 

 the second day of temperature were passed through a meat 

 grinder, and 400 cubic centimeters of a 0.75 per cent phenol 

 solution were added to it. This material was placed in the refrig- 

 erator and thoroughly agitated three or four times a day. After 

 three days' extraction it was filtered through gauze, and the 

 filtrate was returned to the refrigerator, where it was kept until 

 it was 7 days old. The extract was then shipped to the Lubao 

 immunization station for use in the above injections. Of these 

 fifteen animals, seven developed good reactions. Each of these 

 animals received from 400 to 600 cubic centimeters of anti- 

 rinderpest serum, depending on its size, at the same time the 

 extract was injected. One of the reacting animals that received 

 400 cubic centimeters of antirinderpest serum succumbed to the 

 disease, while the others made good recoveries. The animals 

 that did not react were injected with 25 cubic centimeters of 

 virulent blood on September 2, 1917, and one developed the 

 disease from the second injection. There is a possibility that 

 this animal did not contract the disease from the extract injec- 



