156 The Philippine Journal of Science ms 



effects from these injections. They had a slight temperature 

 the day following the injections, which soon subsided to normal. 

 After several days these animals were bled approximately 4 

 liters each. The serum thus obtained has been used in the im- 

 munization stations with good results. 



In view of the results obtained in hyperimmunizing the Chi- 

 nese cattle with the extracts, Doctor Youngberg had the extract 

 tried on some animals in the provincial immunization stations. 

 The extract was prepared at the research laboratory, using liver, 

 spleen, lymphatics, and heart in a 0.75 per cent phenol solution. 

 On the fourth day of extraction this material was filtered through 

 gauze and placed in 15-liter demijohns. When it was 6 days old, 

 it was taken by automobile to the San Fernando and the Apalit 

 immunization stations. On the following day Dr. C. H. Leavitt, 

 in charge of the San Fernando immunization station, injected 

 five animals with 2,000 cubic centimeters each of this extract, 

 and Dr. C. H. Decker, in charge of the Apalit immunization 

 station, injected four animals with 2,000 cubic centimeters each. 



Doctor Leavitt states that a short time after the injection the 

 animals became stiff, stopped eating, and in the course of a few 

 days they presented the appearance of being paralyzed. All 

 five of the animals injected died. Doctor Decker had a some- 

 what similar experience and lost two of the four animals. 



We then tried the same type of extract at the laboratory on 

 two animals that had recently recovered from rinderpest. These 

 animals were each injected with 2,000 cubic centimeters of a 

 7-day-old extract in 0.75 per cent phenol solution. In contrast to 

 the above extracts this material had been kept in the refrigerator 

 up to the time of injection. These animals developed a pro- 

 nounced oedema in the pendent portion of the body, which com- 

 pletely subsided within three days by the aid of slight massaging 

 and the application of warm water each morning. Within four 

 days these animals were in normal condition and never presented 

 any ill effects from the injections up to the time they were bled 

 to death for serum. 



From the results thus obtained it is evident that small Fuga 

 and Batanes cattle can withstand 2,000 cubic centimeters of this 

 extract at one injection without any serious disturbance. 



A further test was made in trying to locate the cause of the 

 losses incurred by Doctors Leavitt and Decker. In this ex- 

 periment the extracts were made in a manner similar to that 

 followed for those described above. On the sixth day they were 

 placed in a demijohn and taken to San Fernando, Pampanga, by 

 automobile and returned to the laboratory the following morning. 



