158 The Philippine Journal of Science 



4. If the extracts are kept at a temperature of approximately 

 15° C, they can be used with safety in 2,000 cubic centimeter 

 doses for hyperimmunization. 



5. Considering our results up to date, the extracts should not 

 be given in massive injections if they have been exposed for a 

 period of eighteen hours to the climatic conditions found in the 

 tropics. 



6. These extracts can be produced so easily that this method 

 can be used in any immunization station. 



7. Considering the similarity of hog cholera to rinderpest, 

 this method should be as applicable in that disease as it is in 

 rinderpest, thereby reducing the enormous cost of the virus. 



REFERENCES 



1. Holmes, J. D. E. Experiments carried out to test the susceptibility of 



cattle from several districts in India, and on improved methods of 

 rinderpest serum production. Indian Civ. Vet. Dept. No. 3, 98—205. 



2. Martoglio, F. On the technique for the production of serum against 



rinderpest. Utilization of the liquid of (blood) vessel lavage as 

 antigen. Memoir of the Eritrea Sero-vaccine Institute. Asmara 

 (1915), No. 1. 



3. Nicolle and Adil-Bey. Etudes sur la peste bovine. Ann. d. I'Inst. 



Pasteur (1901), 715. 



4. Ruediger, E. H. Filtration experiments with virus of cattle plague. 



Phil. Journ. Sci., Sec. B (1908), 3, 165. 



5. Idem. A reduction in the cost of anticattle-plague serum. Ibid., Sec. B 



(1908), 3, 407. 



