COMPARATIVE STUDY ON NATURAL HEMOLYSINS IN INAC- 

 TIVATED HUMAN AND MONKEYS' SERUM 1 



By Carlos Monserrat 

 {From the Serum Section, Biological Laboratory, Bureau of Science, Manila) 



The absence in human serum of natural hemolytic amboceptor 

 toward the red corpuscles of the monkey has been mentioned in a 

 previous communication. 2 



This study suggested the present experiments, in which twenty- 

 three additional samples of human sera were tested with regard 

 to their content of antimonkey natural hemolytic amboceptor. 

 All these samples, showing a lack of antimonkey hemolytic am- 

 boceptor, behaved in the same manner as the forty samples of 

 human sera previously tested. 3 



Kolmer and Casselmann 4 have recently studied the hemoly- 

 sins in inactivated human serum and found that the said serum 

 contains natural hemolysins toward red cells of the following 

 animals: Sheep, dog, calf, goat, pig, rat, chicken, horse, rabbit, 

 and guinea pig. 



On account of our suggestion to substitute, with advantage, 

 monkeys' corpuscles for human red cells in performing the com- 

 plement fixation test for diagnosis of syphilis, it seemed of in- 

 terest to compare human serum with that of the monkey with 

 regard to hemolytic amboceptor toward red cells of some of the 

 animals used by the above-mentioned authors. 



In Table I there are recorded the results obtained by testing 

 twenty-three inactivated human sera and one monkey's serum 

 for hemolysins against human red corpuscles and those of the 

 monkey, the sheep, the horse, the cow, the goat, the carabao, 

 and the guinea pig. 



Technic. — The sera were heated between 55° and 56° C. for 

 thirty minutes. The amount used in the test was 0.2 cubic 



1 Received for publication November, 1917. 



2 This Journal, Sec. B (1917), 12, 249. 



3 Loc. cit. 



4 Journ. Inf. Dis. (1915), 16, 441. 



154722 3 159 



