xiii, b, 4 Wade: Studies on Cryptoplasmic Infection 175 



Small pieces of tissue from three points in the lesion, superfi- 

 cial and deep, were planted (August 17) on nutrient banana 

 agar and on banana cylinders. In smears (October 4) from 

 the material on the latter, bodies of the cell-fragment and basic 

 types were particularly noted, though they were at that time 

 considered insignificant elements of tissue degeneration. 



No further development having occurred, on November 18 

 (three months after planting) the deep-tissue fragment on bana- 

 na cylinder was removed, crushed in a drop or two of sterile 

 bouillon, and distributed to eight slants of special media. These 

 included nutrient prune and nutrient banana agars (some with 

 maltose added) and ordinary 2 per cent maltose and dextrose 

 agars. The last two cultures quickly became overgrown with 

 bacteria, among which very few cell fragments could be found; 

 they were finally discarded as negative (January 3, 1917). In 

 the fruit agar cultures (November 24) numbers of the forms 

 were found, basic forms among them, particularly in the two 

 on nutrient prune. On January 3 they had further increased in 

 these, but were less numerous in the others. The material from 

 several tubes was then transferred to tubes of plain (nonnu- 

 trient) banana agar slants, the parent tubes being kept for fur- 

 ther observation. 



In the subculture from maltose nutrient prune the various 

 forms were numerous on January 17, and at several points 

 in the smear there was a distinct grouping of the basic forms, 

 as if they had multiplied in a mass. The other subplants showed 

 few forms of interest. All were now sealed with paraffin and 

 put away at body temperature. 



On March 11 the smears from parent tubes (from which the 

 November 24 transplants had been made) showed occasional 

 small, round, basic forms on maltose nutrient prune, a very few 

 apparently dividing by an indistinct process of fission. There 

 were a few rounded and oval basic forms and numbers of the less 

 definite cell-fragment bodies on nutrient banana. 



In one nutrient prune agar culture there was found, among 

 the staphylococci and fairly numerous cell-fragment and basic 

 forms, a Cryptococcus essentially similar to that previously found 

 in a culture in case II. In the smear it occurred singly and 

 in small groups (figs. 51 and 52), though sometimes in larger 

 masses (fig. 49). Nothing approaching colony formation could 

 be detected. 



The January 3 subplants now showed less numerous forms of 

 interest than when last examined. Subplants were made from 

 the two most promising of them, and a guinea pig (G. P. 73) 



