176 The Philippine Journal of Science ms 



was inoculated intradermally. Transplants were also made from 

 the Cryptococcus culture to several serum-containing agars. No 

 evidence of further development was observed. On May 28 

 there remained insufficient Cryptococcus-containing material for 

 further study, and all tubes were discarded. 



CASE II 



Ulcers and scars of hands and wrist. — K. M., Filipina, age 20 years, a 

 laundress, seen August 17, 1916. On the back of the left hand was an old 

 depressed scar from an ulcer that had begun two years before and 

 persisted for several months, and in the palm of the right hand (fig. 2) 

 was the scar of an ulcer that healed about a year previously. The active 

 lesions were on the right hand and wrist; dorsally there was an irregular, 

 apparently conglomerate ulcer extending to the fingers, of about one 

 year's duration, apparently involving the deeper structures, the second and 

 third fingers being maintained, though not rigidly, in flexion. Anteriorly 

 on the wrist was another active ulcer, apparently confined to the super- 

 ficial tissues. 



On November 17 the dorsal ulcer (fig. 3) showed little change. The 

 wrist lesion (fig. 2), however, was extending rapidly; above it was a new 

 lesion, but a month old, that was typical, with undermining of the skin at 

 the advancing border, an irregular, granular, very vascular base, and scar 

 formation where the activity had subsided. 



A fragment of tissue excised on August 3 from the wrist ulcer 

 (lower) was planted on a banana-pulp cylinder. It proved to 

 be badly contaminated. On November 17 tissue fragments and 

 scrapings from superficial and deep levels of the newly developed, 

 active ulcer were planted on various media (culture series 1 

 and 2). 



Culture series 1. — This series included six cultures on nutrient 

 banana and nutrient prune agars planted with small tissue frag- 

 ments. On November 25 two were heavily overgrown with molds 

 and bacteria and two others contained little but bacteria; these 

 were discarded. A smear from a deep-tissue fragment on nu- 

 trient banana agar contained numerous small masses and bodies 

 ranging from very pale indefinite fragments to clear-cut, densely 

 stained bodies. Many similar forms were found on nutrient 

 prune agar. The material from the nutrient banana culture 

 was removed and distributed to culture series 3 and to fresh 

 nutrient banana and prune slants ; the latter were made anaerobic. 



Culture series 2. — These cultures, planted simultaneously with 

 series 1 with triturated tissue from deep in the lesion, included, 

 among others, one slant each of: (1) Plain and (2) maltose 

 nonnutrient prune, (3) plain and (4) maltose nutrient prune, 

 (5) plain and (6) maltose nonnutrient banana, and (7) plain 

 and (8) maltose nutrient banana agars. 



