xiii, b, 4 Wade: Studies on Cryptoplasmic Infection 177 



In most of the cultures bacterial colonies appeared, though 

 they developed poorly on the nonnutrient prune and banana 

 agars. In smears from several cultures there were found occa- 

 sional forms more or less approaching the basic type among the 

 bacteria and irregular cell fragments, which were at first rather 

 numerous. On December 17 they were still numerous on several 

 media, though generally diminished. On January 3, 1917, the 

 above described Cryptococcus was found in the culture on nu- 

 trient prune agar (medium 3). This was the first time that it 

 had been observed. On medium 4 there were few of the cell- 

 fragment forms, on 5 and 6 numbers of them were found, and 

 on 1, 2, and 7 they were fairly numerous in the heavy bacterial 

 growth. 



The Cryptococcus was more plentiful on February 6, though 

 nowhere was there a suggestion of colony formation. Various 

 cell-fragment forms were still present in most of the other cul- 

 tures. In culture 6 they seemed to have increased in numbers. 

 In culture 4 some appeared to be attempting to differentiate into 

 the Cryptococcus ; this, however, did not materialize. 



On March 11 the various formed elements having distinctly 

 diminished, transplants were made to other special media, 

 and material containing small numbers of the Cryptococcus was 

 inoculated into a guinea pig (G. P. 44) intradermally and a 

 rabbit (R. 43) intraperitoneal^ in a porous capsule. The re- 

 sults were negative. 



Culture series 3. — On November 25 the material from a nu- 

 trient banana culture (culture series 1) was distributed on the 

 same media as in series 2. The results are of interest only in 

 that, starting with a uniform suspension, the forms here prac- 

 tically disappeared except on media 1 and 5, where they seemed 

 to multiply temporarily. The anaerobic cultures were negative. 



CASE in 



Multiple ulcers of right leg and foot. — L. A., Filipina, age 14, seen August 

 17, 1916. Occupation, housework; seldom in fields. The primary lesion 

 had appeared about a year previously on the right small toe (fig. 5), which 

 had become thickened, decidedly shortened, and was superficially ulcerated. 

 The evident involvement of the deeper tissues, with but a very shallow 

 ulcer, was noteworthy. The consistence of the exposed tissue could not be 

 investigated, the lesion being very sensitive. 



On the inner surface of the leg were two groups of ulcers more or less 

 separated by normal skin and scar tissue. The first had appeared seven 

 months previously. Anteriorly on the ankle was an abruptly raised lesion 

 3 to 4 centimeters in diameter. The skin at the edge was not indurated, 

 but was turned abruptly outward, as if by a draw string, for about 0.8 

 centimeter. The plateau thus produced consisted of granular friable in- 



