188 The Philippine Journal of Science m» 



Figs. 18 and 19. Exudate in superficial necrotic tissue from floor of ulcer. 



Normal-staining polymorphonuclear leucocytes contrasted to 



deeply staining and fragmenting cells. X 500. 

 Fig. 20. Contrasting cell containing two nuclear masses with ordinary, less 



densely stained polymorphonuclear. X 950. 

 Figs. 21 and 22. Cells with three nuclear masses, x 950. 

 Fig. 23. Cell with six nuclear masses (one not visible in this plane) . x 950. 

 Figs. 24 and 25. Groups of small nuclear masses as deposited by breaking 



up of the cell. X 950. 

 Fig. 26. Large cell with six nuclear masses (one not seen), all distinctly 



crescentic (apparently degenerating). X 950. 



27. Two nuclear masses in a leucocyte, density of staining much 



greater than in surrounding cells, x 950. 



28. Two separate bodies, without protoplasm. Whether produced as 



in fig. 27 is not evident. X 950. 

 (Figs. 18 to 28, inclusive, from superficial tissue, case III.) 



Plate IV 



Figs. 29 and 30. Leucocytes separating into deeply staining lobules. 



Smear from culture, case IV, eight days on nutrient prune 



agar. X 1,000. 

 Fig. 31. Leucocyte dividing into three sharply edged fragments, of hyaline 



appearance. X 1,000. 



32. Leucocyte producing two rather deeply staining bodies. X 1,000. 



33. Leucocyte divided into three parts, one becoming oval and deeply 



staining, others soft, diffuse, pale-staining. X 1,000. 



34. Oval body in amorphous remnant of cell. X 1,000. 



Figs. 35 and 36. Soft, amorphous degenerating cells. Clear-cut, round 

 bodies (approaching the basic form) developed elsewhere, x 

 1,00.0. 



Fig. 37. Large body apparently degenerating, x 1,000. 



38. Free-lying body approaching basic. X 1,000. 



(Figs. 31 to 38, inclusive, from smear from culture, case IV, eight 

 days on nutrient banana agar.) 



39. Typical sharply outlined, densely stained basic form. From case 



I, heavily contaminated culture on partially favorable medium. 

 X 840. 



40. Irregular mass of degenerating cell material, quite unlike the basic 



forms. From case I, heavily contaminated culture on unfavor- 

 able medium, x 840. 



Figs. 41, 42, and 43. Leucocytes with deeply staining nuclear masses in 

 smear from tissue fragment, case I, triturated after three 

 months on banana cylinder. Bacterial growth slight. X 840. 

 This material transplanted to nutrient prune agar gave large 

 basic forms; on other media it degenerated more or less 

 promptly. , 



Fig. 44. Two forms approaching basic, apparently derived from one cell. 

 X 840. 



45. Clear-cut basic form, x 840. 



46. Irregular body (approaching basic type) and very small clear-cut 



body suggestive of an organism, x 840. 



