xm, b, 4 Wade: Studies on Cryptoplasmic Infection 189 



Plate V 



Figs. 47 and 48. Groups of more or less clear-cut, deeply stained cell 

 fragments and basic forms. X 840. 

 (Figs. 44 to 48, inclusive, from case II, culture of tissue frag- 

 ments eight days on nutrient prune agar.) 



Fig. 49. Masses of cryptococci, case I. Culture of tissue fragment three 

 months on banana cylinder and four months on nutrient prune 

 agar. X 1,000. 

 50. Scattered cryptococci, case II. Culture of tissue fragments six 

 weeks on nutrient prune agar. 



Figs. 51 and 52. Small groups of the cryptococcus, case I, same smear as 

 fig. 49. Showing density of staining and irregularity in size 

 and outline. X 1,150. 



Fig. 53. Cryptococci, case II, from same culture as fig. 50. Showing 

 (arrow) apparent attempt at mycelial growth. (This offshoot 

 in the smear is clearly distinct from the surrounding bacteria.) 

 X 1,300. 



Figs. 54, 55, and 56. Showing irregularity of size, shape, and mode of 

 multiplication, neither clear-cut budding nor simple binary fis- 

 sion. X 1,150. 



Fig. 57. Group of four sharply pointed apple-pip-like cryptococci. Their 

 shape and close aggregation suggest origin by fission in two 

 planes. X 1,150. 



Figs. 58 and 59. Cryptococci apparently degenerating, becoming pale stain- 

 ing in the former and unstained in the latter. Spaces believed 

 not to represent organisms dropped out of smears. X 1,000. 

 (Figs. 54 to 59, inclusive, from same smear as fig. 53.) (Cul- 

 tural forms all stained with Loeffler's methylene blue.) 



