278 



The Philippine Journal of Science 



1918 



vibrios in the faeces was considerable, but when the amount of 

 cholera vibrios in the faeces was decreased, the ox bile proved 

 to be superior to the sodium chloride solution (Tables IV and V). 



CONCLUSIONS 



1. Glycerin, sodium chloride solution, and ox bile were tested 

 for their value as preservatives of cholera faeces for delayed 

 bacteriological examination. 



2. Glycerin was found unfit for that purpose, while sodium 

 chloride solution and bile gave good results. 



3. Ox bile was found superior to sodium chloride solution in 

 those instances where the cholera vibrios were present in the 

 faeces in. small numbers. 



Table I. — Showing the preserving action of glycerin on cholera vibrios. 



PLATE FROM PEPTONE. 



Immediately 



24 hours 



48 hours 



72 hours 



Fourth day . 

 Fifth day. __ 

 Sixth day... 





Glycerin. 





Sodium 

 chloride. 



20 per 

 cent. 



25 per 

 cent. 



30 per 

 cent. 



+ 



+ 



+ 



+ 



+ 



H- 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



+ 



- 



+ 



+ 



+ 



— 



+ 

 + 



+ 



- 



- 





Table II. — Showing the preserving action of salt solution on cholera vibrios. 



Concentration of salt solution. 



0.5 per cent __ + 



1 per cent ._ + 



2 per cent - 



5 per cent 



10 per cent + 



15 per cent _ + 



20 per cent I + 



25 per cent + 



30 per cent _ _J + 



Control (normal salt solution) + 



Dieudonn^'s plate. 



In days. 



In weeks. 



12 



4 5 



•2 3 4 5 



