﻿200 
  REPORT 
  UNITED 
  STATES 
  ENTOMOLOGICAL 
  COMMISSION. 
  

  

  and 
  Anabrus 
  is 
  identical 
  with 
  that 
  observed 
  in 
  other 
  insects, 
  271 
  for 
  which 
  

   I 
  refer 
  the 
  reader 
  to 
  Waldeyer, 
  272 
  Leydig, 
  273 
  and 
  particularly 
  to 
  the 
  elabo- 
  

   rate 
  and 
  accurate 
  memoir 
  of 
  Dr. 
  Ludwig. 
  274 
  

  

  I 
  wish 
  to 
  describe 
  somewhat 
  carefully 
  the 
  relation 
  of 
  the 
  egg 
  to 
  the 
  cells 
  

   of 
  the 
  follicular 
  wall 
  in 
  the 
  locust. 
  If 
  a 
  bit 
  of 
  the 
  wall 
  of 
  one 
  of 
  the 
  largest 
  

   follicles 
  be 
  spread 
  out 
  on 
  a 
  slide 
  and 
  colored 
  with 
  carmine 
  it 
  will 
  be 
  seen 
  

   that 
  it 
  is 
  composed 
  of 
  very 
  large 
  and 
  beautiful 
  cells, 
  Fig. 
  20. 
  The 
  cells 
  

   are 
  for 
  the 
  most 
  part 
  pentagonal 
  in 
  outline, 
  a 
  few 
  being 
  hexagonal. 
  The 
  

   nuclei 
  are 
  very 
  large, 
  taking 
  up 
  a 
  great 
  part 
  of 
  the 
  cells, 
  in 
  the 
  center 
  

   of 
  which 
  they 
  lie. 
  They 
  are 
  slightly 
  oval, 
  though 
  departing 
  but 
  little 
  

   from 
  a 
  circular 
  outline. 
  They 
  are 
  darkly 
  colored 
  by 
  carmine, 
  and 
  are 
  

   filled 
  with 
  innumerable 
  small 
  granules, 
  which 
  prevented 
  my 
  ascertaining 
  

   whether 
  there 
  is 
  any 
  nucleolus, 
  though 
  in 
  many 
  cases 
  there 
  seemed 
  to 
  

   be 
  one 
  of 
  considerable 
  size. 
  A 
  transverse 
  section 
  through 
  the 
  whole 
  

   follicle, 
  such 
  as 
  is 
  reproduced 
  in 
  Fig. 
  19, 
  shows 
  that 
  the 
  cells 
  form 
  a 
  

   single 
  continuous 
  layer, 
  Ep, 
  around 
  the 
  egg. 
  Eg. 
  Examined 
  with 
  a 
  higher 
  

   power, 
  Fig. 
  16, 
  such 
  a 
  section 
  reveals 
  the 
  form 
  of 
  the 
  cells. 
  The 
  free 
  or 
  

   outer 
  surface 
  of 
  the 
  epithelium, 
  Ep 
  y 
  is 
  nearly 
  flat, 
  while 
  the 
  side 
  towards 
  

   the 
  egg. 
  Eg, 
  is 
  dome-shaped. 
  Between 
  the 
  epithelium 
  and 
  the 
  egg 
  there 
  

   is 
  a 
  layer, 
  &h, 
  of 
  finely- 
  granulated 
  and 
  very 
  pale 
  substance 
  that 
  is 
  not 
  

   colored 
  by 
  carmine 
  or 
  h 
  sematoxiline. 
  This 
  layer 
  has 
  its 
  outer 
  surface 
  

   hollowed 
  out 
  into 
  little 
  cups, 
  each 
  of 
  which 
  is 
  intended 
  to 
  receive 
  the 
  

   dome-shaped 
  end 
  of 
  one 
  of 
  the 
  epithelial 
  cells, 
  as 
  is 
  shown 
  very 
  plainly 
  

   in 
  Figs. 
  16 
  and 
  19, 
  which 
  represent 
  sections 
  in 
  which 
  the 
  epithelium 
  is 
  

   artificially 
  raised 
  from 
  t 
  he 
  granular 
  layer. 
  In 
  the 
  normal 
  condition 
  the 
  

   cells 
  rest 
  directly 
  on 
  the 
  layer, 
  and 
  there 
  is 
  no 
  clear 
  space, 
  as 
  indicated 
  

   in 
  Fig. 
  16. 
  It 
  is 
  to 
  be 
  added 
  that 
  the 
  layer 
  in 
  question 
  consists 
  of 
  three 
  

   strata: 
  1, 
  a 
  very 
  delicate 
  external 
  membrane, 
  which 
  rests 
  against 
  the 
  

   epithelium 
  ; 
  2, 
  the 
  middle 
  granular 
  portion 
  ; 
  and 
  3, 
  a 
  fibrous 
  stratum, 
  

   which 
  assumes 
  a 
  roseate 
  hue 
  after 
  staining 
  with 
  carmine, 
  and 
  which 
  lies 
  

   next 
  the 
  egg. 
  The 
  layer 
  is 
  formed 
  in 
  the 
  follicles, 
  and 
  is 
  probably 
  se- 
  

   creted 
  by 
  the 
  follicular 
  epithelium. 
  It 
  is 
  not 
  to 
  be 
  found 
  in 
  the 
  upper 
  

   part 
  of 
  the 
  ovarian 
  tube. 
  If 
  I 
  am 
  not 
  mistaken, 
  it 
  passes 
  over 
  with 
  the 
  

   egg 
  into 
  the 
  oviduct, 
  being 
  destined 
  to 
  form 
  part 
  of 
  the 
  shell. 
  I 
  cannot 
  

   help 
  suspecting 
  that 
  it 
  is 
  this 
  structure 
  which 
  has 
  given 
  rise 
  to 
  the 
  opin- 
  

   ion 
  that, 
  at 
  least 
  in 
  some 
  insects, 
  the 
  wall 
  of 
  the 
  egg 
  follicle 
  passes 
  off 
  

   with 
  the 
  egg 
  to 
  form 
  part 
  of 
  the 
  shell. 
  

  

  In 
  the 
  egg 
  proper 
  of 
  locusts, 
  Figs. 
  16 
  and 
  19, 
  Eg, 
  the 
  enormous 
  masses 
  

   of 
  nutritive 
  yolk 
  deserve 
  special 
  mention, 
  though 
  I 
  have 
  been 
  unable 
  

   to 
  determine 
  their 
  relation 
  to 
  the 
  protoplasm 
  of 
  the 
  egg. 
  

  

  The 
  ovarian 
  tubes 
  have 
  further 
  an 
  external 
  tunic, 
  which 
  I 
  have 
  studied 
  

  

  271 
  Excellent 
  diagrams 
  of 
  the 
  development 
  of 
  eggs 
  in 
  insects 
  are 
  given 
  in 
  Gogenbaur's 
  Grnndziige 
  der 
  

   Vergleichendon 
  Anatomie. 
  2 
  Aufl., 
  p. 
  4G3, 
  fig. 
  121, 
  and 
  an 
  even 
  bettor 
  figure 
  of 
  Vanessa 
  urticce 
  is 
  given 
  

   by 
  Waldeyer 
  in 
  Strieker's 
  Handbuch 
  der 
  Lebro 
  von 
  don 
  Geweben, 
  p. 
  5G3, 
  fig. 
  195. 
  

  

  272 
  Waldeyer: 
  Eierstock 
  und 
  Ei, 
  Leipzig 
  (1870), 
  p. 
  86, 
  and 
  especially 
  p. 
  90. 
  

  

  27S 
  Leydig: 
  Eierstock 
  und 
  Samentascbo 
  der 
  Insecten. 
  Nov. 
  Act. 
  Caes. 
  Leop., 
  xxxiii 
  (18G7). 
  

   874 
  H. 
  Ludioig 
  : 
  Sompor's 
  Arboiten. 
  Bd. 
  I. 
  

  

  