22 



Krperiinful •!. 1(X) cc. of a snliition of iimltom- coiilikiiiin^ 1.324 >?rm. of 

 poliil inattor, luul llit- tnuivalriit of OSfj.') ;;rin. kIiicok-, wito inixtil with l<X)c.c. 

 of !i Icoji Solution coiitaiiiiii;^ IJ..'>72 'grains of noli.l.s tiiul 2.14 j^rni». >jIiilxin', ami 

 licated for 2i lioiii-s to IJ.I^OC Tlic litjiiiil after liiatiiig (llie koji Ix-'itifj dr- 

 (luctod) gave in KX) c.c. 1.374 gram Foliils nn«l 1.348 gram {I'r.coM;. It i« 

 fvidont, tlierefoic, (liat tlic Folntioii of niiilloff liad Utn coni|>lit(ly coiivcrled 

 into <lo.\trosi'. 'I'lie ]iro|ioiti"!i of koji Folntion t:H'd in tliis CN|>tTiin('tit waa very 

 large. 



Experiment •/. A solution of maltose was |iri'i>;in.'d containing 2.08 gnimx 

 of Solid matter in 100 c.c , and giving an o]iticjil rotation in a 20() mm. tulie nf 

 :}2.1 divi.sions, c<iiial to a spirific rotntory [Miwer |«|, = 144^..'). 100 c.c. of tliiK 

 Solution were mi.\ed with KX) c.c. of koji extract containing 2.3 grm«. of noliii 

 matter in 100 c.c. ami giving in a 200 mm. tnl>e an o|>tical rotation of lO.fi divi- 

 sions. This mixture WiU halted to GO^O. for 2J hr.s. on the water-l«th, then 

 cooled and diluted to '2')1 c.c. at l.')^J. It c.Mitiinel 2 03 gr.imi of soli«! 

 matter in KW c.c. and gave an optical rotation of 11.5 division«. Deducting the 

 amount of solids due to 100 c.c. of koji extract in 250 c.c. wo gut as tiie n.-su)t 

 iif the atlio'i u|)on the maltose— 



Siliil m.itter 2.775 gnuna. 



Opticjil rotation 7.3 divinions. 



(4 ■?»'•<'. 



The action of koji cxtniot in rctlucing the 8|iccific rotitory i>owtr of maltoso 

 is thus very marked. The explanation of the n.f<luction of course, is, that 2.CS 

 g/.uns of maltose having a s|)ecilic rotatory jiower equal to 144^.5 have taken tip 

 0.005 gnim water forming dextrose having a specific rotitory jMuver c.pial to 59". 

 The numl)or I'.f .G shows that the hydrating action was not (juitc comph'te, an«l 

 this is confirmetl hy the quantity of water alisorlK»!, which for 2f>8 grams of 

 maltose ought to have Iwen 0.14 gr. 



The following exiieriment will allow us to tr;ice the gnulual action of the 

 koji solution ui>on the nialtose takiii'^ jls the standard of compirisoii the s|«'cific 

 rotatory power. 



Ejpeylmiiit ö. KX) C.c. of the «üme s<dnti(>n of maltoite as wiui used in tlie 

 last exjieriment were mixed with 100 cc. of a freshly pn;|iorcd extract of koji, 

 which contairiLHl 2.424 grams of »olid m.itter in lOO c.c. ami which gave an 

 cfiticnl rotation in a 20) mtn. ttilieof 11 divisions. '1 he mixtimt of maltow and 

 koji solutions WiLS diluted to 500 c.c. at 15^^C, and afti-r stnnding at that tetn- 

 perattirc for 10 mintttes a sample was withdrawn for analysis. The rcm.iinder 

 was ](laced in a water Kith hcateil to 4.>''(/'. and sampln« were t^tkeii after the 

 lap-c of 3U mill., 1 hr., and 2 hrs. 



