Ol 



From those exfieriments wc may nndei^tniil '.vhat ocfur? during; the mi\shing 

 ojierations in «xkö makintr. At first tlicy are conducted at even a lower 

 temjieratiire than -l^C. and tliis iö iiiteiv-stiiig lieoause it siiows th it the iiotivity 

 of the diastase in köji is not destroye«! -at low teiiijxjmtures, even at 0°C. 



At a liiiiher l«'m|H'n\tnre the rwlurtion in tlie sjiocitic rotatory p<nver of the 

 M>lution goes on more rapidly, and althona;!! the sake-hrewer never uses teui- 

 ]XTaturv'S so hi^h as those of the succeetlins: experiments, it is of scientific interest 

 to complete the niord at all tetnperatures l)elow that at which the "diastase" 

 of köji is rendei^ed inartive. 



The experinieiits at higher temiieratures were conducted in the lollowing 

 manner. The fiisk containing tlie mixture was immersed in a water-bath and 

 kept at the spocifie<l temperature, samples licing taken at stated intervals. The 

 jiortion used for determining the tofcil solid matter in solution from its speci- 

 fic gravity was nxpidly cot)led by means of ice, and another portion in which the 

 specific rotatory jMiwer was to lie determined, was poure<l into a dry flask con- 

 taining a little salicylic acid, as recomraendetl by Brown and Heron,"' and also 

 rapidly cooled. Induction was made for the amount of köji solution added as 

 in the previous experiments. 



The two next serii's of experiments were conducted at 40*^0. and only ditfer 

 in the relative pro{K>rtions of starch and köji u.se<l. 



TABLE XV. ACTION OF KÖJI EXTRACT UPON STARCH AT 40°C. 

 10 grams of starch to 5 grams of koji. 



Timo. 



Total st.ircli products ' 

 in solution i 

 (knji deduct««!) 



Specific rotatory 



power of 

 starch products. 



—I 



20 min 



10.08 grams. . 



1.- i 



4 hours 



10.08 ., 



127= 



-l-n-i hrs. at Vt'C 



10.-25 



106° 



TABLIC XVI. ACTION OK KÖJI KXTK.VCT UPON STARCH AT JO°C. 

 10 grams of starch to 10 graius of köji. 



Tin 



Totjil stJirch products . Siiecifir rotatory 



in solution 

 (kflji dodni'i'd ' 



power ot 



,1. 



j" hour 





9.t)4 



i»a .1 



1 





«.(54 



127° 



2 hour^ 





9.0.4 



115° 



8 





9.05 



106° 



4J „ 





9.07 



HR" 



«; 





ü.fiO 



8i-.° 



4 20 hrs. nt lo'C. 





9.7« 



80° 



loc. cit. 1879. Tran», p. « 



ISO. 







J 



