730 EXPERIMENTS ON "CATTANACH DISINFECTANT AND DEODORANT," 



that had been derived from a mesentei'ic gland of an undoubted 

 case of typhoid fever, and had been transferred from gelatine to 

 gelatine during a period of somewhat less than a year. It did 

 not in any way differ from the Bacillus typhi ahdomincdis of 

 Eberth-Gaffky. Of the purity of the above culture I convinced 

 myself by means of Koch's plate-process ; from the isolated 

 typical colonies fresh gelatine-tubes were sown. 



The arrangement of the experiments was as follows : — In a first 

 series I inoculated with the bacillus some small Erlenmeyer's 

 flasks containing ordinary meat-broth of a slightly alkaline 

 reaction,* and placed in a thermostat for two days at a 

 temperature of 32-3 1:°C. After this period the nourishing liquid 

 had assumed a suitable degree of turbidity. I then mixed the 

 contents of all the flasks together, and filled a number of sterilised 

 1 oz. = 28 ccm. flasks, each with 10 ccm. of the infected broth 

 (conf. p. 736). These were then charged with certain quantities 

 of the "Oattanach powder," and in a parallel manner with the 

 " Carbolic powder." Tests for ascertaining the effect of these 

 substances were made from time to time, as will be seen from the 

 table below. I may here at once state that in these experiments as 

 well as in the following ones, liquid and powder, immediately after 

 the addition of the latter, were carefully mixed with one another, 

 then allowed to stand at the temperature of the room undisturbed 

 till a test was to be made, when they were stirred up again, and 

 so on. A flask containing the respective liquid without any 

 addition served for control-experiments each time. The same 

 platinum-loop was used in all cases for transferring minute samples 

 of the test-material (one loop full) to an 8-10 p.c. nutrient gelatine 

 (slightly alkaline), that having been liquefied for that purpose at 

 40°C., and less, was made to solidify along the inner walls of the 



•The same as used in the preparation of nutrient gelatine, agar-agar, etc., 

 without the addition of any further substance. 



