BY DR. OSCAR KATZ. 731 



test-tube (after Esmarch). The test-tubes were kept under 

 observation for at least a fortnight at ordinary temperatures. 



Whether bacteria grew in the culture-tubes or not, was seen by 

 the appearance or non-appearance of colonies in them. That, 

 speaking of the experiments with typhoid bacilli, the colonies 

 which made their appearance were in fact such of the named 

 bacillus, was proved by their macroscopic and microscopic examina- 

 tion ; it must also be borne in mind that the object under con- 

 sideration was a pure culture of a well-characterised micro-organism. 

 The presence of a minute portion of the two kinds of disinfect- 

 ants, as transferred along with the broth to the culture tubes, 

 did not in any case prevent the bacillus from developing ; in case 

 of the culture-medium remaining sterile from the very first, it was 

 inoculated with living culture-material of the same bacillus, when 

 after sometime a normal growth of the organism could be noticed. 



A second series of experiments on the typhoid-bacillus differed 

 in its arrangement from the former, in so far as the culture- 

 medium for the bacillus was meat-broth that had not been 

 deprived of the flocculent and rather tenacious masses of 

 coagulated albumen, which is formed by boiling the beef-infusion. 

 Such a nutritive soil resembling as it were artificial typhoid dejecta, 

 has been made use of before by Liborius.* Of this material 25 gr. 

 each were filled into a number of sterilised Erlemneyer's flasks, 

 inoculated with a minute and equal quantity of a pure-culture and 

 allowed to stand in the thermostat for two days, at about the 

 same temperature as before. Afterwards they were charged with 

 definite and proportionally the same quantities, as before, of the 

 two powders to be examined. 



In the appended tables (pp. 736-737) I give an account of the 

 arrangement and of the results of the above experiments. 



*P. LiboriuH, Einige Untersuchungen iiber die deainficirende Wirkung 

 des Kalkes. Zeitschr. f. Hygiene. Bd. II., Heft 1, Leipzig, 1887, p. 41. 



