112 



WALKER. 



liquor was used. Tliere wei'e required 0.12 cubic centimeter — potassium 

 hydroxide for neutralization. 



Conclusion. — No enzyme eapaljle of hj'drolizlng ethyl biityrate is i^res- 

 ent in the press liquor from the coconut foot. 



For comparison I give one experiment by Kastle and Loevenhart ^ 

 working with a 10 per cent extract obtained from the pancreas of a pig. 



One cubic centimeter extract, 4 of water, 0.26 of ethyl butyrate and 

 0.1 of toluol were kept at 40° for fifteen minutes and showed an increase 



■ N 

 in acidity corresponding to 1.63 cubic centimeter — potassium hydroxide. 



A similar test with the boiled extract showed no increase in acidity 

 whatsoever. 



My Experiment I was continued as follows : 



(e) Eight cubic centimeters of fresh liquor, 5 of coconut oil and 0.1 of toluol 



N 



were allowed to stand one week. Eight cubic centimeters of potassium 



10 

 hydroxide were required for neutralization. 



(/) Eiglit cubic centimeters of hoiled liquor, 5 of coconut oil, and 0.1 of 



toluol were* allowed to stand one week. 19.9 cubic centimeters potassium 



10 ' 

 hydroxide were required for neutralization. 



The boiled liquor showed a considerably greater acidity on standing 

 than did the fresh, hence it is evident that no hydrolysis by enzymes had 

 thus far been proved. The nut from which this foot was taken was 

 perfectly sound and free from mold, but the inner surface of the meat 

 next to the foot had l}egun to soften and had a greasy feel. A portion of 

 this softened meat was dried and expressed, yielding an oil containing 

 3.3 j)er cent of free fatty acids, showing that hydrolysis to a marked 

 extent had taken place in the growing nut. 



COCONUT MEAT. 



Experiment II. — The sprouting nut used for this series contained a foot 

 whicli almost filled it. Tlie meat remaining was ground in a sausage grinder and 

 a cream-like emulsion pressed out. 



(o) The action with ethyl hutyrate.—rThe conditions were; 5 cubic centi- 

 meters of water, 0.25 of ethyl butyrate, 1 of toluol and 1 of cream, with the 

 following result: 



Conditions. 



11 hours at 

 40° C. 



Neutralized, let 



stand at room 



temperature 



1 day. 



Let stand 1 day 

 more at room 

 tempera- 

 ture. 



Not neutralized, 

 room temper- 

 ature for 

 2 days. 





0. "15 cc. ^ KOH 

 0.35cc.^KOH 



0.12CC. ^KOH 

 O.lOec.^g 



O.lOcc. I^KOH 0.92CC. ^KOH 



Boiled cream ___ 



0. lU cc. ~ KOH 



10 

 0.4oce.^KOH 



'Amer. Chem. .Journ. (1900) 24, 491. 



