114 



WALKER. 



MEAT AND MILK. 



Ea:periincnt I\\ — (a) Tbe expressed cream from tlie meat of a nut just 

 beginning to sprout was used, tlie conditions l)eing as follows: 



Five cubic centimeters of water, 0.25 of etliyl butyrate, 1 of toluol and 1 

 of cream. 



Conditions. 



At start. 



Neutral- 

 ized, and 

 let stand 

 24 hours. 



Not neutralized. 



Koom 

 tempera- 

 ture 24 

 hours. 



1 

 Incubator 24 

 hours. 



a. 



b. 



Unboiled 



0. 47 cc. ^ KOH 

 0. 47 CC. 



cc. 

 0.18 

 0.16 



cc. 

 1.82 

 0.70 



cc. 

 1.24 

 0.60 



cc. 

 2.00 

 0.47 



Boiled 





(6) Five cubic centimeters of water, 0,25 of ethyl butyrate, 1 of 1 to 1,000 



formalin and 1 of cream were used. . The unboiled mixture after twenty-four 



N 

 hours at room temperature took 0.55 cubic centimeter of potassium hydroxide 



and the boiled mixture 0.48 to neutralize. 



Here again, although the miboiled cream iiicrea.ses in acidity fairly 

 raj)idly in the presence of toluol, the increase nm.st be due chiefly to 

 inefficient antiseiDsis, as it is almost entirelj^ inhibited by formalin in a 

 dilution of 1 to 7,000. Experiment has shown that formalin of this 

 strength has jjractically no action on enzymes. 



Experiment V. — The cream used in Experiment IV was treated with alcohol 

 and ether, the precipitate washed with alcohol and finally with ether and then 

 dried in a vacuum over sulphuric acid. One gram of tliis powder rubbed up 

 with 20 cubic centimeters of water yielded a milky liquor wbicli was tested for 

 the presence of enzymes, the following being used : 



Five cubic centimeters of water, 0.25 of ethyl butyrate, 1 of toluol and 1 of 

 the liquor, the mixture being kept in the incubator at 35° to 40° for two days. 



N 



io 



Both the boiled and unboiled liquor required 0.8 cubic centimeter of 



potassium hydroxide to neutralize. 



It is evident that the precipitate obtained from coconut cream by the 

 above method contains no fat-splitting enzjanes. 



COCONUT FOOT AND COCONUT OIL. 



Experiment VI. — (a) One hundred grams of foot from two nuts with sprouts 

 about 1 meter long were mixed in a mortar with 100 cubic centimeters of fresh 

 coconut oil, 100 of water and 1 of chloral. The mixture was well ground for 

 about one hour to prepare a good emulsion, it was then strained through cloth 

 and 25 cubic centimeter portions were placed in small, stoppered Erlenmeyer 



