78 The Philippine Journal of Science wib 



isolated, further attempts to obtain the glucoside in a pure state 

 were discontinued. Nothing definite regarding the amount of 

 the glucoside originally present can be adjudged from the small 

 quantity of material isolated, since nothing is known concerning 

 the previous history of the nuts received. They had the appear- 

 ance of having been exposed to the weather ; consequently hydrol- 

 ysis of the glucoside due to the activity of the enzyme probably 

 occurred. Fresh seeds have been requested in order that a quan- 

 titative determination of the amount of glucoside present can 

 be made. 



The water and chloroform portions were combined, acidified 

 with hydrochloric acid, and subjected to steam distillation. The 

 distillate gave tests for acetic and formic acids. 



COMPOSITION OP THE OIL 



One hundred grams of the oil were saponified with alcoholic 

 potash, the alcohol was distilled off, and the dried mass was ex- 

 tracted with petroleum ether. An oil was extracted which on 

 recrystallization from alcohol possessed a melting point of 132° 

 C. and gave all the reactions of phytosterol. 



For a complete examination of the fatty acids 500 grams of 

 the oil were saponified with alcoholic potash and the acids were 

 freed in the aqueous solution by the addition of acid. The free 

 acids were then extracted with ether. A white solid mass was 

 obtained with the following values: A melting point of 43° C, 

 an iodine value of 102.9, a saponification value of 203.3, and 

 specific rotation of +60.96°. The mixture of free acids was dis- 

 solved in alcohol with warming, and on cooling the part sepa- 

 rating out was removed. After recrystallization from alcohol 

 and ethylacetate it had a melting point of 68° C, identical with 

 the melting point of the chaulmoogric acid found by Power and 

 Barrowcliff " in the oil from Taraktogenos kurzii, H. anthel- 

 minticus, and H. wightiana. A solution of 2.071 grams in 50 

 cubic centimeters of chloroform produced in a 2-decimeter tube 



a rotation of +4.95°, whence [a] ^ =+59.75°. 



2.1409 grams required 76.16 cubic centimeters N/10 NaOH for 

 neutralization. Theoretical, volume N/10 alkali for neutralization 

 of 2.1408 grams ChHsiCOOH requires 76.38 cubic centimeters. 



0.7990 gram of the sodium salt gave 0.1862 gram NajSO*, whence 

 Na = 7.55 per cent. Theoretical for Ci,H,iCOONa = 0.1878 gram 

 NajSOi, or Na = 7.62 per cent. 



0.2029 gram absorbed 0.1833 gram iodine, whence I = 90.32 per cent. 

 CuHuOa with one ethylenic linking requires I = 90.60 per cent. 



"Loc. cit. 



